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Vectorized data acquisition and fast triple-correlation integrals for Fluorescence Triple Correlation Spectroscopy.

William K Ridgeway¹, David P Millar, James R Williamson

¹Dept. of Molecular Biology, The Scripps Research Institute, 10550 N. Torrey Pines Rd., La Jolla CA 92037, USA ; Dept. of Chemistry, The Scripps Research Institute, 10550 N. Torrey Pines Rd., La Jolla CA 92037, USA ; The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 N. Torrey Pines Rd., La Jolla CA 92037, USA.

Computer Physics Communications

|March 26, 2013

Summary

This summary is machine-generated.

We developed the Triple Correlation Toolbox for Fluorescence Triple Correlation Spectroscopy (F3CS). This software enables advanced analysis of molecular interactions and dynamics using existing microscopes.

Keywords:

Data Acquisition F3CS FCS Fluorescence Fluorescence Triple Correlation Spectroscopy Multiple-tau

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Area of Science:

Biophysics
Spectroscopy
Computational Biology

Background:

Fluorescence Correlation Spectroscopy (FCS) is a key technique for quantifying molecular dynamics and concentrations.
Existing methods like FCS have limitations in analyzing complex mixtures and irreversible processes.

Purpose of the Study:

To introduce the Triple Correlation Toolbox, a computational suite for Fluorescence Triple Correlation Spectroscopy (F3CS).
To enable advanced analysis of molecular stoichiometries and time-reversal asymmetries.

Main Methods:

Development of a complete data analysis pipeline for acquiring, correlating, and fitting large datasets.
Implementation of accelerated data acquisition using counter-timer chips and vectorized operations.
Utilizing a two-delay time multiple-tau scheme for efficient correlation integral calculation.

Main Results:

The Triple Correlation Toolbox provides accurate error estimates for global fitting.
Accelerated data acquisition allows sub-microsecond resolution for extended periods on desktop computers.
The toolbox facilitates fitting FCS and F3CS data for models with up to ten species.

Conclusions:

The Triple Correlation Toolbox is a comprehensive software package that makes F3CS accessible on standard microscopes.
This advancement allows for detailed analysis of complex molecular interactions and dynamics.