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Updated: May 13, 2026

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates
Published on: May 9, 2025
Engineering effective T-cell based antitumor immunity
Thomas Gruber1, Reinhard Hinterleitner, Christa Pfeifhofer-Obermair
1Department of Pharmacology and Genetics; Medical University Innsbruck; Innsbruck, Austria.
Abstract:
The adoptive transfer of synthetic siRNA-mediated cblb-depleted autologous CD8+ T cells acts as a potent adjuvant for dendritic cell (DC) vaccination and provides a significant therapeutic benefit. Our proof-of-concept study validates the strategy of inhibiting CBLB as a rational approach to augment the effectiveness of adoptively transferred immune cells.
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