Related Experiment Video
Updated: May 12, 2026
13:37
Polymer Microarrays for High Throughput Discovery of Biomaterials
Published on: January 25, 2012
Selective serial multi-antibody biosensing with TOPAS microstructured polymer optical fibers
Grigoriy Emiliyanov1, Poul E Høiby, Lars H Pedersen
1DTU Fotonik, Department of Photonics Engineering, Technical University of Denmark, Kgs. Lyngby, Denmark. egrigoriy@hotmail.com
Sensors (Basel, Switzerland)
|March 27, 2013
Summary
Researchers created a novel fiber-optic biosensor for detecting multiple antibodies. This innovative optical biosensor uses fluorescence to selectively identify different antibodies within a single fiber.
Area of Science:
- Biomedical Engineering
- Optical Sensors
- Materials Science
Background:
- Selective antibody detection is crucial for diagnostics.
- Existing biosensing methods can be limited in multiplexing capabilities.
- Microstructured polymer optical fibers offer unique platforms for sensing applications.
Purpose of the Study:
- To develop a fluorescence-based fiber-optic biosensor for serial detection of antibodies.
- To demonstrate selective sensing of different antibodies at distinct locations within a single fiber.
- To utilize UV activation for localized sensor layer functionalization.
Main Methods:
- Fabrication of a microstructured polymer optical fiber using TOPAS cyclic olefin copolymer.
- Localized UV activation of sensor layers within the fiber's microstructures.
- Serial fluorescence-based detection of Cy3-labelled α-streptavidin and Cy5-labelled α-CRP antibodies.
Main Results:
- Successful development of a fiber-optic biosensor capable of selective antibody detection.
- Demonstration of serial sensing of two different antibodies (α-streptavidin and α-CRP) at preselected positions.
- Validation of UV activation for creating localized sensing regions within the polymer fiber.
Conclusions:
- The developed fiber-optic biosensor enables multiplexed antibody detection in a single fiber.
- This technology offers a promising platform for advanced diagnostic and sensing applications.
- The use of TOPAS polymer and UV activation provides a versatile method for creating localized biosensors.
