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Related Concept Videos

Patch Clamp01:18

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...
Potentiometry: Types of Electrodes01:19

Potentiometry: Types of Electrodes

Reference electrodes serve as a stable reference point for potentiometric measurements, while indicator and working electrodes react to variations in the composition of a solution.
The Standard Hydrogen Electrode (SHE) is a widely used reference electrode that maintains zero potential across all temperatures. However, its need for a continuous hydrogen gas supply renders it impractical for everyday use.
An alternative to SHE is the Saturated Calomel Electrode (SCE). This electrode features an...
Potentiometry: Membrane Electrodes01:15

Potentiometry: Membrane Electrodes

Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at the...
Controlled-Potential Coulometry: Electrolytic Methods01:17

Controlled-Potential Coulometry: Electrolytic Methods

Controlled-potential coulometry, also known as potentiostatic coulometry, employs a three-electrode system in which the working electrode's potential is precisely regulated using a potentiostat. Platinum working electrodes are utilized for positive potentials, while mercury pool electrodes are favored for extremely negative potentials. The platinum counter electrode is separated from the analyte using a membrane or salt bridge to avoid interference in the analysis.
The chosen potential ensures...
Standard Electrode Potentials03:02

Standard Electrode Potentials

On comparing the reactivity of silver and lead, it is observed that the two ionic species, Ag+ (aq) and Pb2+ (aq), show a difference in their redox reactivity towards copper: the silver ion undergoes spontaneous reduction, while the lead ion does not. This relative redox activity can be easily quantified in electrochemical cells by a property called cell potential. This property is commonly known as cell voltage in electrochemistry, and it is a measure of the energy which accompanies the charge...
Voltage Doubler Circuit01:23

Voltage Doubler Circuit

A voltage doubler circuit integrates two main components: a clamping section and a rectifier section. The clamping section consists of a capacitor (C1) and a diode (D1), whereas the rectifier section is equipped with another diode (D2) and capacitor (C2). This circuit produces an output voltage with twice the amplitude of the sinusoidal input voltage.

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Related Experiment Video

Updated: May 12, 2026

Single Cell Measurement of Dopamine Release with Simultaneous Voltage-clamp and Amperometry
07:30

Single Cell Measurement of Dopamine Release with Simultaneous Voltage-clamp and Amperometry

Published on: November 21, 2012

Two-electrode voltage clamp.

Bingcai Guan1, Xingjuan Chen, Hailin Zhang

  • 1The Key Laboratory of Neural and Vascular Biology, Ministry of Education, Shijiazhuang, Hebei Province, China.

Methods in Molecular Biology (Clifton, N.J.)
|March 27, 2013
PubMed
Summary
This summary is machine-generated.

Two-electrode voltage clamp (TEVC) is a key electrophysiology method for studying ion channels. This study details using TEVC with Xenopus oocytes and mRNA expression for effective ion channel recording.

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Area of Science:

  • Electrophysiology
  • Molecular Biology
  • Biophysics

Background:

  • Two-electrode voltage clamp (TEVC) is a standard technique for controlling cell membrane potential.
  • It is crucial for studying electrogenic membrane proteins, particularly ion channels.
  • Accurate measurement of membrane potential and current is essential for understanding channel function.

Purpose of the Study:

  • To describe the application of TEVC for ion channel recording.
  • To demonstrate the combined use of TEVC with exogenous mRNA expression in Xenopus oocytes.
  • To provide a methodology for studying ion channel properties in a controlled experimental setting.

Main Methods:

  • Utilizing two intracellular electrodes for voltage sensing and current injection.
  • Implementing TEVC to maintain a precise membrane potential (V m).
  • Expressing exogenous mRNA in Xenopus oocytes to study specific ion channels.

Main Results:

  • TEVC successfully controlled membrane potential in Xenopus oocytes.
  • Ion channel activity was accurately recorded using the combined approach.
  • The method allows for detailed analysis of ion channel kinetics and function.

Conclusions:

  • TEVC combined with exogenous mRNA expression in Xenopus oocytes is a powerful tool for ion channel research.
  • This technique facilitates the study of specific ion channel properties.
  • The described method enhances the understanding of electrogenic membrane protein function.