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Related Concept Videos

Patch Clamp01:18

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

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Related Experiment Video

Updated: May 12, 2026

Application of Automated Image-guided Patch Clamp for the Study of Neurons in Brain Slices
09:05

Application of Automated Image-guided Patch Clamp for the Study of Neurons in Brain Slices

Published on: July 31, 2017

Automated planar patch-clamp.

Carol J Milligan1, Clemens Möller

  • 1Florey Institute of Neuroscience and Mental Health, Melbourne Brain Centre, Kenneth Myer Building, Royal Parade, Melbourne, VIC, Australia.

Methods in Molecular Biology (Clifton, N.J.)
|March 27, 2013
PubMed
Summary
This summary is machine-generated.

Automated planar patch-clamp technology offers high-throughput, efficient, and reliable ion channel assays. This advanced method enables detailed studies of ion channel function in both cell lines and native cells, aiding drug discovery and research.

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Last Updated: May 12, 2026

Application of Automated Image-guided Patch Clamp for the Study of Neurons in Brain Slices
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Area of Science:

  • Biophysics
  • Molecular Biology
  • Pharmacology

Background:

  • Ion channels are crucial membrane proteins regulating ion transport in biological systems.
  • Dysfunction of ion channels is implicated in numerous diseases, making them key therapeutic targets.
  • Manual patch-clamping has been the gold standard for ion channel research, but is time-consuming.

Purpose of the Study:

  • To highlight breakthroughs in automated planar patch-clamp technology for ion channel research.
  • To present the features and protocols of the Nanion NPC-16 Patchliner platform.
  • To demonstrate the applicability of automated patch-clamp systems in drug discovery and academic research.

Main Methods:

  • Development and application of automated planar patch-clamp systems.
  • Utilizing high-throughput platforms for ion channel assays.
  • Employing techniques for simultaneous perfusion and fast compound application.

Main Results:

  • Automated platforms provide high-quality data with enhanced efficiency and reliability.
  • Newer systems successfully study ion channels in native and primary mammalian cells.
  • Platforms like Nanion's Patchliner offer flexibility for high-throughput studies.

Conclusions:

  • Automated planar patch-clamp technology significantly advances ion channel research capabilities.
  • This technology streamlines drug discovery and academic investigations.
  • The described protocols are adaptable to various automated patch-clamp platforms.