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Patch Clamp

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

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Related Experiment Video

Updated: May 12, 2026

Preparation of Drosophila Central Neurons for in situ Patch Clamping

Preparation of Drosophila Central Neurons for in situ Patch Clamping

Published on: October 15, 2012

Patch-clamping Drosophila sensory neurons.

Volodymyr Kucher¹, Benjamin A Eaton, James D Stockand

¹Department of Physiology, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.

Methods in Molecular Biology (Clifton, N.J.)

|March 27, 2013

Summary

This summary is machine-generated.

Researchers developed a new method to study ion channel activity in fruit fly (Drosophila) sensory neurons using patch-clamp electrophysiology. This technique identifies the pickpocket1 (Ppk1) channel as responsible for sensing acidic conditions.

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Last Updated: May 12, 2026

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Area of Science:

Neuroscience
Molecular Biology
Genetics

Background:

Electrophysiology is crucial for understanding ion channel function.
Studying ion channels in vivo in model organisms like Drosophila can be challenging.
Drosophila are powerful genetic tools for physiological research.

Purpose of the Study:

To develop and present a novel method for recording ion channel activity in Drosophila sensory neurons.
To demonstrate the utility of this method for studying ion channel function in different genetic backgrounds.
To identify the specific ion channel responsible for acid-sensing in Drosophila sensory neurons.

Main Methods:

Preparation of primary neuronal cell cultures from Drosophila embryos.
Patch-clamp electrophysiology (whole-cell current-clamping and voltage-clamping) of cultured Drosophila sensory neurons.
Analysis of sodium (Na+) currents and neuronal excitability.
Genetic manipulation of Drosophila.

Main Results:

The patch-clamp method successfully recorded ion channel activity in cultured Drosophila sensory neurons.
Whole-cell current-clamping revealed action potential firing in multidendritic (md) sensory neurons.
Voltage-clamping identified the voltage-gated sodium channel, paralytic.
Acidic pH activated acid-sensing inward Na+ currents in class IV md neurons.
Genetic analysis identified the pickpocket1 (Ppk1) channel as responsible for these acid-sensing currents.

Conclusions:

Primary neuronal cultures from Drosophila embryos provide a viable platform for electrophysiological studies of ion channels.
The developed patch-clamp technique allows detailed analysis of neuronal excitability and ion channel function.
The pickpocket1 (Ppk1) channel is identified as a key mediator of acid-sensing in Drosophila class IV md sensory neurons.