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Both proteoglycans and collagen are attached to fibronectin proteins, which, in turn, are attached to integrin proteins. These integrin proteins interact with transmembrane...

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Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy

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Published on: August 20, 2018

Fibronectin fixation on poly(ethyl acrylate)-based copolymers.

N Briz¹, C M Antolinos-Turpin, J Alió

¹Tecnalia Research & Innovation, Health Division-Biomaterials Area, Mikeletegi Pasealekua 2, 20009 Donostia-San Sebastian, Spain.

Journal of Biomedical Materials Research. Part B, Applied Biomaterials

|March 27, 2013

Summary

This study quantifies fibronectin (FN) adhesion and cell-binding motif exposure on modified poly(ethyl acrylate) (PEA) copolymers. Hydrophilic surfaces enhanced the exposure of key cell adhesion sequences, crucial for cell proliferation.

Keywords:

acrylic acid fibronectin adsorption grafting hydroxyethyl acrylate methacrylic acid poly(ethyl acrylate)

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Wet Chemistry and Peptide Immobilization on Polytetrafluoroethylene for Improved Cell-adhesion

Wet Chemistry and Peptide Immobilization on Polytetrafluoroethylene for Improved Cell-adhesion

Published on: August 15, 2016

Area of Science:

Biomaterials Science
Surface Chemistry
Cell Biology

Background:

Fibronectin (FN) is a crucial extracellular matrix protein mediating cell adhesion and proliferation.
Understanding protein adsorption and biomotif exposure on polymer surfaces is vital for designing biocompatible materials.
Poly(ethyl acrylate) (PEA) copolymers offer tunable properties for biomaterial applications.

Purpose of the Study:

To quantify fibronectin (FN) adhesion and the exposure of its cell-adhesive motifs (RGD and FNIII7-10) on PEA copolymers.
To investigate the impact of copolymer composition, wettability, and functional groups on FN interaction.
To correlate FN motif exposure with cell adhesion and proliferation potential.

Main Methods:

Synthesis of PEA copolymers with varying hydrophilic content and acid functional groups.
Adsorption and covalent grafting of FN onto PEA copolymer surfaces.
Quantification of adhered FN using enzyme-linked immunosorbent assay (ELISA).
Assessment of RGD and FNIII7-10 motif exposure via ELISA.

Main Results:

Copolymers exhibited decreased water contact angles, indicating increased wettability.
FN successfully adhered to all tested surfaces, with higher adhesion on hydrophobic surfaces.
Despite higher adhesion on hydrophobic surfaces, hydrophilic PEA copolymers showed significantly greater exposure of FN cell adhesion sequences (RGD and FNIII7-10).

Conclusions:

Surface wettability is a critical factor influencing the presentation of cell-adhesive motifs on FN-coated biomaterials.
Hydrophilic PEA copolymers promote higher exposure of essential FN cell adhesion sites, suggesting enhanced potential for cell adhesion and proliferation.
Tailoring PEA copolymer chemistry offers a promising strategy for developing advanced biomaterials with improved cellular interactions.