Related Experiment Video
Updated: May 12, 2026
10:21
Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix
Published on: June 14, 2016
Using proteomics to uncover extracellular matrix interactions during cardiac remodeling
Nicolle L Patterson1, Rugmani Padmanabhan Iyer, Lisandra E de Castro Brás
1San Antonio Cardiovascular Proteomics Center, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.
Proteomics. Clinical Applications
|March 28, 2013
Summary
Myocardial infarction triggers left ventricle (LV) remodeling. Proteomics, including matridomics and degradomics, reveals matrix metalloproteinases
Area of Science:
- Cardiovascular Biology
- Proteomics
- Molecular Medicine
Background:
- Myocardial infarction (MI) initiates a complex cardiac remodeling process in the left ventricle (LV).
- This remodeling involves cardiomyocyte death, extracellular matrix (ECM) deposition, and scar formation.
- Leukocytes and cardiac fibroblasts orchestrate the wound healing response post-MI.
Purpose of the Study:
- To review the application of proteomics in understanding the role of matrix metalloproteinases (MMPs) in post-myocardial infarction LV remodeling.
- To highlight the significance of matridomics and degradomics in studying ECM and protease dynamics.
Main Methods:
- This review synthesizes existing research utilizing proteomic techniques.
- Focuses on studies employing matridomics (ECM proteomics) and degradomics (protease and substrate analysis).
Main Results:
- Matrix metalloproteinases (MMPs) are key regulators of inflammatory and ECM responses during cardiac scar formation.
- Proteomic analyses provide detailed insights into MMP activity and substrate modification post-MI.
- Understanding these MMP roles is crucial for modulating scar quality and LV function.
Conclusions:
- Proteomics offers powerful tools to dissect the intricate roles of MMPs in LV remodeling after myocardial infarction.
- Targeting MMPs presents a potential therapeutic strategy to improve outcomes following heart attack.
- Further proteomic research can refine our understanding of cardiac repair mechanisms.
More Related Videos
Related Concept Videos
01:42The Extracellular Matrix
Overview
01:29The Extracellular Matrix
Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
01:24Overview of Cell-Matrix Interactions
The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
01:33Proteomics
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
01:23Role of Matrix Metalloproteases in Degradation of ECM
Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult body.
A...
A...
01:13Cell-matrix's Response to Mechanical Forces
In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue.
Anchoring junctions mechanically attach a cell to the...
Anchoring junctions mechanically attach a cell to the...