Molecularly imprinted SPE combined with dispersive liquid-liquid microextraction for selective analysis of
Mohana Krishna Reddy Mudiam1, Abhishek Chauhan, Amit Kumar Singh
1Analytical Chemistry Section, CSIR-Indian Institute of Toxicology Research, M G Marg, Lucknow, 226 001, UP, India. mohanitrc@gmail.com
Background:
The present communication describes the combination of molecularly imprinted SPE and dispersive liquid-liquid microextraction for the selective preconcentration and determination of telmisartan (TEL) in rat urine, plasma and pharmaceutical formulation by HPLC.
Results:
Various factors that can affect the extraction efficiency of molecularly imprinted SPE and dispersive liquid-liquid microextraction were optimized. The LOD and LOQ were found to be 0.19 and 0.63 µg ml(-1) in urine, while in plasma it was found to be 0.28 and 0.87 µg ml(-1), respectively. The percentage recovery of TEL in different matrices was found to be in the range of 81-97%.
Conclusion:
The proposed method may find wide applications in clinical, toxicological and QC laboratories for the routine analysis of TEL.
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