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Updated: May 1, 2026
Isolation of Pulmonary Artery Smooth Muscle Cells from Neonatal Mice
Published on: October 20, 2013
Mesenchymal stem cells protect against neonatal rat hyperoxic lung injury
Hongshan Zhang1, Jianpei Fang, Yanyun Wu
1Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University, Department of Pediatric , Yanjiang Road 107, Guangzhou, Guangdong 510120 , China. zhhosh@126.com
Objectives:
Bronchopulmonary dysplasia (BPD) is a significant global health problem and currently lacks effective therapy. We established a neonatal rat model of BPD to investigate therapeutic potential of bone marrow-derived mesenchymal stem cells (BMSCs) in neonatal hyperoxic lung injury.
Methods:
BMSCs were isolated, identified, and transfected by lentiviral vector carrying green fluorescent protein gene in vitro. Neonatal Sprague-Dawley rats were injected intravenously with either BMSCs or phosphate-buffered saline following 95% oxygen exposure, and assessed for the survival rate and alveolar injury during recovery.
Results:
Treatment with BMSCs after oxygen exposure for 7 days improved survival of neonatal rat during recovery. BMSCs protected against neonatal rat hyperoxic lung injury during recovery as demonstrated by enhanced expression of AQP5 and SP-C, likely due to the suppression of alveolar cell apoptosis and lung inflammation responses to oxygen with up-regulation of the expression of BCL-2 gene and down-regulation of the expression of BAX gene and stimulation of vascular endothelial growth factor and so on.
Conclusions:
BMSCs protect against O2-mediated injury partially through stimulation of potent mediators that participate in tissue repair.
