Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An interpretable deep learning framework uncovers features governing CRISPR-Cas9 genome-editing efficiency.

Bioinformatics (Oxford, England)·2026
Same author

Pharmacists in modern primary care and management of diseases: an evidence-based perspective on emerging technologies.

BioImpacts : BI·2026
Same author

Emerging advancements and expanding technological scope of education and practices in pharmacy and pharmaceutical sciences.

BioImpacts : BI·2026
Same author

Recent developments in intein-mediated protein splicing and their applications in bioengineering.

Current protein & peptide science·2026
Same author

Bridging Preclinical and Clinical Gaps in Ocular Therapeutics: Hydrogel Drug Delivery and 3D Tissue Models.

International journal of nanomedicine·2026
Same author

Biomarker-driven and AI-assisted nanomedicine for breast cancer: advancing precision drug delivery from bench to bedside.

Expert opinion on drug delivery·2026

Related Experiment Video

Updated: May 12, 2026

Therapeutic Gene Delivery and Transfection in Human Pancreatic Cancer Cells using Epidermal Growth Factor Receptor-targeted Gelatin Nanoparticles
08:35

Therapeutic Gene Delivery and Transfection in Human Pancreatic Cancer Cells using Epidermal Growth Factor Receptor-targeted Gelatin Nanoparticles

Published on: January 4, 2012

EGFR antisense oligonucleotides encapsulated with nanoparticles decrease EGFR, MAPK1 and STAT5 expression in a human

Ahmad Gholamhoseinian Najar1, Roghiyeh Pashaei-Asl, Yadollah Omidi

  • 1Department of Biochemistry and Kerman Physiology Research Center, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.

Asian Pacific Journal of Cancer Prevention : APJCP
|March 29, 2013
PubMed
Summary

This study shows that PAMAM nanoparticles effectively deliver EGFR antisense oligonucleotides to HT29 cancer cells, significantly reducing epidermal growth factor receptor (EGFR) expression and downstream signaling pathways.

More Related Videos

Development and Maintenance of a Preclinical Patient Derived Tumor Xenograft Model for the Investigation of Novel Anti-Cancer Therapies
09:29

Development and Maintenance of a Preclinical Patient Derived Tumor Xenograft Model for the Investigation of Novel Anti-Cancer Therapies

Published on: September 30, 2016

Related Experiment Videos

Last Updated: May 12, 2026

Therapeutic Gene Delivery and Transfection in Human Pancreatic Cancer Cells using Epidermal Growth Factor Receptor-targeted Gelatin Nanoparticles
08:35

Therapeutic Gene Delivery and Transfection in Human Pancreatic Cancer Cells using Epidermal Growth Factor Receptor-targeted Gelatin Nanoparticles

Published on: January 4, 2012

Development and Maintenance of a Preclinical Patient Derived Tumor Xenograft Model for the Investigation of Novel Anti-Cancer Therapies
09:29

Development and Maintenance of a Preclinical Patient Derived Tumor Xenograft Model for the Investigation of Novel Anti-Cancer Therapies

Published on: September 30, 2016

Area of Science:

  • Oncology
  • Nanotechnology
  • Molecular Biology

Background:

  • Epidermal growth factor receptor (EGFR) overexpression is common in many human cancers.
  • Targeting EGFR is a promising strategy for cancer therapy.

Purpose of the Study:

  • To investigate the efficacy of EGFR antisense oligonucleotides delivered via PAMAM nanoparticles.
  • To evaluate the impact of EGFR inhibition on downstream signaling pathways (MAPK, STAT3) in HT29 cells.

Main Methods:

  • Utilized FITC-labeled EGFR antisense oligonucleotides encapsulated in PAMAM nanoparticles.
  • Assessed cellular uptake using fluorescence microscopy and flow cytometry.
  • Quantified EGFR mRNA and protein levels via real-time PCR and Western blotting.

Main Results:

  • PAMAM nanoparticle-encapsulated antisense effectively reduced EGFR mRNA and protein expression in HT29 cells.
  • EGFR inhibition led to a decrease in EGFR-dependent downstream gene expression.
  • Confirmed successful cellular uptake of the antisense oligonucleotides.

Conclusions:

  • EGFR antisense oligonucleotides delivered by PAMAM nanoparticles represent a viable strategy for downregulating EGFR.
  • This approach effectively inhibits EGFR and its associated signaling pathways, offering potential for cancer treatment.