Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Acetobacter cellulose pellicle as a temporary skin substitute.

J D Fontana1, A M de Souza, C K Fontana

  • 1LQBB-Biomass Chemio/Biotechnology Lab, Department of Biochemistry, UFPR, Curitiba-PR, Brazil.

Applied Biochemistry and Biotechnology
|January 1, 1990
PubMed
Summary

Researchers cultured a bacterial strain similar to Acetobacter xylinum to produce high-purity cellulose pellicles. These bacterial cellulose biofilms show promise as effective biological dressings for various skin wound treatments.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Response Letter to 'Optimising physiology for adolescents with dysautonomia'.

Acta paediatrica (Oslo, Norway : 1992)·2017
Same author

Exercise and the multidisciplinary holistic approach to adolescent dysautonomia.

Acta paediatrica (Oslo, Norway : 1992)·2017
Same author

First record of Stenochrus portoricensis Chamberlin, 1922 (Arachnida: Schizomida: Hubbardiidae) for the Pernambuco state, Brazil.

Brazilian journal of biology = Revista brasleira de biologia·2015
Same author

Toxicological in vitro and subchronic evaluation of LASSBio-596.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association·2014
Same author

Response to: echocardiography assessment of the aorta in children with chronic kidney disease.

Pediatric cardiology·2014
Same author

"Extended Fitness" hypothesis: a link between individual and group selection.

Genetics and molecular research : GMR·2013

Area of Science:

  • Microbiology and Biomaterials Science

Background:

  • Acetobacter xylinum is known for producing bacterial cellulose.
  • Cellulose pellicles have potential applications in medicine.
  • Developing efficient methods for producing high-quality cellulose is crucial.

Purpose of the Study:

  • To culture a bacterial strain for producing thick, smooth cellulosic pellicles.
  • To assess the cellulose content and nature of the produced pellicles.
  • To evaluate the potential of the bacterial cellulose biofilm as a biological dressing.

Main Methods:

  • Culturing a bacterial strain resembling Acetobacter xylinum in a nonagitated, inverted sucrose- and yeast water-based medium.
  • Assessing cellulose content (>90% dry weight) and beta-D-homopolyglucan nature using physical, chemical, and enzymatic methods.

Related Experiment Videos

  • Inactivating bacterial biomass (BioFill) with ethylene dioxide for safe application.

    • Main Results:

    • Successfully produced thick, smooth, floating cellulosic pellicles with high cellulose content.
    • Confirmed the beta-D-homopolyglucan structure of the pellicles.
    • Demonstrated that the inactivated biofilm (BioFill) has advantages as a biological dressing.

    Conclusions:

    • The cultured bacterial strain effectively produces high-purity bacterial cellulose pellicles.
    • The resulting biofilm (BioFill) is apyrogenic and suitable for medical applications.
    • Bacterial cellulose biofilms are valuable as temporary skin substitutes for treating skin wounds like burns and ulcers.