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Published on: February 1, 2018
Development of biosensor for phenolic compounds containing PPO in β-cyclodextrin modified support and iridium
Cristiano P da Silva1, Ana C Franzoi, Suellen C Fernandes
1Departamento de Química, Laboratório de Biossensores, Universidade Federal de Santa Catarina, 88040-970, Florianópolis, SC, Brazil.
Enzyme and Microbial Technology
|April 2, 2013
Summary
A novel biosensor utilizing iridium nanoparticles and celery polyphenol oxidase (PPO) was developed for sensitive rutin detection. This electrochemical sensor offers reliable quantification of phenolic compounds in simulated samples.
Area of Science:
- Electrochemistry
- Biosensor Technology
- Analytical Chemistry
Background:
- Phenolic compounds, like rutin, are important antioxidants with diverse biological activities.
- Accurate detection of phenolic compounds is crucial for food quality control and health assessments.
- Electrochemical biosensors offer a sensitive and selective platform for analyzing bioactive molecules.
Purpose of the Study:
- To construct and optimize an electrochemical biosensor for the detection of phenolic compounds.
- To utilize iridium nanoparticles and polyphenol oxidase (PPO) from celery for enhanced sensing capabilities.
- To determine the optimal conditions for rutin detection using square-wave voltammetry.
Main Methods:
- Fabrication of a biosensor using iridium nanoparticles dispersed in an ionic liquid (Ir-BMI·PF6) and celery PPO immobilized on a β-cyclodextrin modified support (β-CDEP).
- Investigation of phenolic compound behavior using square-wave voltammetry.
- Optimization of sensor composition, enzyme concentration, buffer conditions, and voltammetric parameters.
Main Results:
- Rutin was identified as the phenolic compound with the greatest signal response.
- Optimized conditions yielded a linear response for rutin in the concentration range of 1.3×10⁻⁷–2.0×10⁻⁶ M, with a detection limit of 7.9×10⁻⁸ M.
- The biosensor demonstrated good repeatability, reproducibility, and high recovery rates (92.8–103.4%) with a low relative error (0.7%) in simulated samples.
Conclusions:
- The developed Ir-BMI·PF6/PPO/β-CDEP biosensor is effective for the sensitive and selective determination of rutin.
- The sensor exhibits excellent analytical performance, making it suitable for practical applications in sample analysis.
- This work highlights the potential of combining nanomaterials, ionic liquids, and enzymatic systems for advanced biosensing.
