Polysaccharide extraction from Spirulina sp. and its antioxidant capacity
Ratana Chaiklahan1, Nattayaporn Chirasuwan, Panya Triratana
1Pilot Plant Development and Training Institute, King Mongkut's University of Technology Thonburi, Bang Khun Thain, Bangkok 10150, Thailand. ratana.cha@kmutt.ac.th
Abstract:
To optimize polysaccharide extraction from Spirulina sp., the effect of solid-to-liquid ratio, extraction temperature and time were investigated using Box-Behnken experimental design and response surface methodology. The results showed that extraction temperature and solid-to-liquid ratio had a significant impact on the yield of polysaccharides. A polysaccharides yield of around 8.3% dry weight was obtained under the following optimized conditions: solid-to-liquid ratio of 1:45, temperature of 90°C, and time of 120 min. The polysaccharide extracts contained rhamnose, which accounted for 53% of the total sugars, with a phenolic content of 45 mg GAE/g sample.
