Silver nano particles prevent platelet adhesion on immobilized fibrinogen

Vascular thrombotic disorders have emerged as a serious threat to our society. Platelet adhesion to fibrinogen, collagen and other platelet activators exposed over the atherosclerotic plaques can trigger platelet signaling events, activate platelets and lead to thrombotic events. Since anticoagulant and thrombolytic treatment strategies are usually associated with serious bleeding complications, preventing platelets adhesion may help to maintain platelets in an inactive state. In this study we tried to find out the effect of Silver nanoparticles, through their interaction with various platelet surface integrins on platelet adhesion on immobilized fibrinogen. Platelets, isolated from anti-coagulated human whole blood sample from healthy donors, were suspended in physiological buffer and each sample was divided into four tubes. In three of them 0.05, 0.5, and 5 μM concentrations of Silver nanoparticles were added, fourth tube served as control. Platelet adhesion on immobilized fibrinogen matrices and integrin mediated cell signaling events were studied in all the four samples. In the present study we show that nanosilver prevent platelet adhesion without conferring any lytic effect on them and effectively prevents integrin-mediated platelet responses in a concentration-dependent manner.