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High-Performance Liquid Chromatography: Instrumentation

High-Performance Liquid Chromatography: Instrumentation

High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.

High-Performance Liquid Chromatography: Introduction

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:

High-Performance Liquid Chromatography: Elution Process

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...

Ion-Exchange Chromatography

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...

High-Performance Liquid Chromatography: Types of Detectors

High-Performance Liquid Chromatography: Types of Detectors

The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...

Supercritical Fluid Chromatography

Supercritical Fluid Chromatography

Supercritical fluid chromatography (SFC) provides a beneficial substitute for gas chromatography (GC) and liquid chromatography (LC) for certain samples because it merges the top attributes of both techniques. SFC allows the separation and analysis of compounds that GC or LC does not easily manage. These compounds are traditionally nonvolatile or thermally unstable, making GC unsuitable and lacking functional groups required for HPLC analysis.
SFC utilizes a supercritical fluid mobile phase,...

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Updated: May 12, 2026

$Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems$

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

Published on: September 23, 2021

An Advanced, Interactive, High-Performance Liquid Chromatography Simulator and Instructor Resources.

Paul G Boswell¹, Dwight R Stoll, Peter W Carr

¹University of Minnesota, 1970 Folwell Ave., St. Paul MN, 55108.

Journal of Chemical Education

|April 2, 2013

Summary

This summary is machine-generated.

A new, free, open-source High-Performance Liquid Chromatography (HPLC) simulator offers sophisticated features for education. This web-based tool provides accessible training and immediate feedback for diverse users, addressing limitations of existing software.

Keywords:

Analytical Chemistry Chromatography Computer-Based Learning Graduate Education HPLC Internet / Web-Based Learning Upper-Division Undergraduate

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Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)

Published on: March 14, 2013

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$Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems$

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

Published on: September 23, 2021

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)

Published on: March 14, 2013

Area of Science:

Analytical Chemistry
Computational Chemistry

Background:

Existing High-Performance Liquid Chromatography (HPLC) simulation software is often expensive, outdated, or lacks essential features for effective educational use.
There is a need for accessible, comprehensive HPLC simulation tools for students and educators.

Purpose of the Study:

To develop and introduce a free, open-source, web-based HPLC simulator.
To provide an accessible and sophisticated tool for learning and teaching HPLC principles and experimental design.

Main Methods:

Development of a web-based simulation platform for HPLC.
Implementation of intuitive controls for various experimental parameters (e.g., mobile phase composition, flow rate, column type).
Integration of a graphical chromatogram display for real-time feedback on parameter changes.

Main Results:

The developed HPLC simulator is free, open-source, and accessible online at hplcsimulator.org.
The simulator features sophisticated yet user-friendly controls and provides immediate visual feedback via chromatogram generation.
Positive feedback was received from students using the simulator in an undergraduate Analytical Chemistry course.

Conclusions:

The new free, open-source HPLC simulator effectively addresses the limitations of existing tools.
This simulator serves as a valuable and accessible educational resource for a wide range of users in HPLC.
The platform includes example problem sets to facilitate integration into chemistry curricula.