Related Experiment Video
Updated: May 12, 2026

20:00
Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
Published on: October 31, 2015
Demonstration of a plenoptic microscope based on laser optical feedback imaging.
Wilfried Glastre1, Olivier Hugon, Olivier Jacquin
1Centre National de la Recherche Scientifique / Université de Grenoble 1, Laboratoire Interdisciplinaire de Physique, UMR 5588, Grenoble, France. wilfried.glastre@ujf-grenoble.fr
Optics Express
|April 3, 2013
Summary
A novel plenoptic imaging system using Laser Optical Feedback Imaging (LOFI) offers enhanced performance over microlens arrays. This new system demonstrates improved resolution and depth of field for advanced microscopy applications.
Area of Science:
- Optics and Photonics
- Microscopy
- Imaging Systems
Background:
- Plenoptic imaging, also known as light field imaging, captures spatial and angular information of light rays.
- Traditional plenoptic microscopes often utilize microlens arrays for light field capture.
- Limitations in photometric performance and resolution exist in current plenoptic microscopy techniques.
Purpose of the Study:
- To introduce and evaluate a new plenoptic imaging system based on Laser Optical Feedback Imaging (LOFI).
- To compare the performance of the LOFI-based plenoptic system against existing microlens array devices.
- To demonstrate the theoretical and experimental capabilities of the LOFI system for advanced microscopy tasks.
Main Methods:
- Development of a novel plenoptic imaging system incorporating Laser Optical Feedback Imaging (LOFI).
- Comparative analysis of the LOFI system against a standard microlens array-based plenoptic device.
- Theoretical modeling and experimental validation of plenoptic properties, including numerical refocusing and aberration compensation.
Main Results:
- The LOFI-based plenoptic system achieved superior photometric performance, resolution, and depth of field compared to the microlens array system.
- The trade-off for improved performance was a slower point-by-point scanning acquisition method.
- Key plenoptic microscope properties, such as refocusing on curved surfaces and aberration correction, were successfully demonstrated.
Conclusions:
- Laser Optical Feedback Imaging (LOFI) presents a viable and effective approach for developing advanced plenoptic imaging systems.
- The LOFI-based plenoptic microscope offers significant advantages in image quality and flexibility for scientific imaging.
- Further development may focus on optimizing scanning speed to fully leverage the enhanced imaging capabilities.
Related Concept Videos
Confocal Fluorescence Microscopy
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Imaging Biological Samples with Optical Microscopy
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

