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Updated: May 12, 2026

Asymmetrical Flow Field-Flow Fractionation for Sizing of Gold Nanoparticles in Suspension
Published on: September 11, 2020
Separation science: Principles and applications for the analysis of bionanoparticles by asymmetrical flow field-flow
Alexandre Moquin1, Françoise M Winnik, Dusica Maysinger
1Faculty of Pharmacy, and Department of Pharmacology & Therapeutics, Faculty of Medicine, Université de Montréal and McGill University, Montreal, QC, Canada.
Abstract:
Field-flow fractionation is an analytical technique that allows the separation of particles over a size range, from a few nanometers to several microns in diameter. The separation takes place under mild conditions and is suited for the analysis of neutral or charged particles. A single measurement yields the size and concentration of each component of a mixture. However, developing a suitable fractionation method can be tedious and time-consuming. In this chapter, we present asymmetrical flow field-flow fractionation (AF4) conditions that have proven their reliability for the analysis of quantum dots and other nanoparticles in the 5-50 nm size range. Common pitfalls are emphasized together with strategies to overcome them.
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