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Related Concept Videos

Genetic Screens02:46

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...

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Updated: May 12, 2026

Competitive Genomic Screens of Barcoded Yeast Libraries
11:59

Competitive Genomic Screens of Barcoded Yeast Libraries

Published on: August 11, 2011

Screening of genomic libraries.

Valdenice M Novelli1, Mariângela Cristofani-Yaly, Marinês Bastianel

  • 1Instituto Agronômico, Centro APTA Citros Sylvio Moreira, Cordeirópolis, SP, Brazil.

Methods in Molecular Biology (Clifton, N.J.)
|April 3, 2013
PubMed
Summary
This summary is machine-generated.

Microsatellite (SSR) markers are crucial for plant genetics and breeding. This study presents an efficient method for developing SSR markers from genomic DNA, particularly useful for species lacking existing databases.

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Last Updated: May 12, 2026

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Area of Science:

  • Plant Genetics
  • Molecular Biology
  • Genomics

Background:

  • Microsatellites, also known as simple sequence repeats (SSRs), are vital molecular markers in plant genetics and breeding.
  • Current methods for SSR marker development rely on genomic DNA or expressed sequence tags (ESTs).
  • SSR markers offer high polymorphism, codominance, and conserved sequences, driving their increased use in genetic studies.

Purpose of the Study:

  • To describe an efficient methodology for developing microsatellite markers using enriched genomic DNA libraries.
  • To provide a valuable tool for plant species lacking ESTs or genome databases.

Main Methods:

  • Development of microsatellite markers through enrichment of genomic DNA libraries.
  • Application of the methodology to Citrus spp. for SSR marker library enrichment.

Main Results:

  • The described methodology is highly efficient for developing SSR markers.
  • This approach is particularly beneficial for plants without available ESTs or genome databases.
  • Enriched SSR marker libraries were successfully generated for Citrus spp.

Conclusions:

  • The genomic enrichment method provides an efficient strategy for developing SSR markers.
  • This technique is crucial for genotyping germplasm, selecting zygotic hybrids, and saturating genetic maps in plant breeding programs.
  • It offers a valuable alternative for species with limited genomic resources.