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Updated: May 12, 2026

Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA
Published on: June 25, 2014
Simple sequence repeats amplification
Kundapura V Ravishankar1, Padmakar Bommisetty
1Division of Biotechnology, Indian Institute of Horticultural Research, Bangalore, India.
Abstract:
The technique of SSR amplification is a prerequisite to generate the molecular profiles of various alleles of an individual or genotype. Amplification is the multifold duplication and accumulation of a targeted region which is achieved by polymerase chain reaction. It needs ingredients such as buffer, MgCl2, dNTPs, primers, and DNA polymerase enzyme. The utilization of these essential PCR components in optimal concentrations determines the success of amplification. Thus SSRs, as primers, play an important role in enhancing the amplification and thereby generating the genotype profile. With the advent of technology, fluorophore-labeled primers along with automated capillary electrophoresis system have enhanced the efficiency of detection.
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