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Related Concept Videos

DNA Isolation01:34

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

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Related Experiment Video

Updated: May 12, 2026

Isolation of Genomic DNA from Mouse Tails
07:26

Isolation of Genomic DNA from Mouse Tails

Published on: July 29, 2007

DNA isolation from mammalian samples.

Ximeng Liu1, Shuko Harada

  • 1Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama, USA.

Current Protocols in Molecular Biology
|April 3, 2013
PubMed
Summary
This summary is machine-generated.

This study compares DNA isolation methods, including silica spin columns and phenol/chloroform extraction, for fresh and FFPE tissues. Silica spin columns are currently the most common technique for DNA extraction.

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Area of Science:

  • Molecular Biology
  • Genetics

Background:

  • DNA isolation is crucial for molecular genetic studies.
  • Various DNA extraction techniques exist, each with advantages and disadvantages.

Purpose of the Study:

  • To describe and compare two primary DNA isolation methodologies: silica spin column and phenol/chloroform extraction.
  • To present basic DNA extraction procedures for both fresh and formalin-fixed, paraffin-embedded (FFPE) tissue samples.

Main Methods:

  • Silica spin column-based DNA isolation.
  • Phenol/chloroform DNA extraction.
  • DNA extraction from fresh and FFPE tissues.

Main Results:

  • Silica spin column technique is the most commonly used method for DNA isolation.
  • Phenol/chloroform extraction remains a widely utilized technique.
  • Basic procedures for DNA extraction from diverse tissue types are detailed.

Conclusions:

  • Both silica spin column and phenol/chloroform methods are effective for DNA isolation.
  • The choice of method depends on specific research needs and sample types.
  • Standardized procedures are essential for reliable DNA extraction from various tissue sources.