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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.

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Related Experiment Video

Updated: May 12, 2026

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay
06:15

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay

Published on: September 7, 2018

Quantitative serum immunoglobulin tests.

Richard K S Loh1, Sandra Vale, Andrew McLean-Tooke

  • 1Immunology Department, Princess Margaret Hospital for Children, Subiaco, Western Australia. richard.loh@health.wa.gov.au

Australian Family Physician
|April 4, 2013
PubMed
Summary
This summary is machine-generated.

Quantitative serum immunoglobulin tests measure IgG, IgA, and IgM protein levels to aid in diagnosing diseases. Abnormal levels can indicate various health conditions, including deficiencies or excesses.

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Last Updated: May 12, 2026

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay
06:15

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Published on: September 7, 2018

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Quantitative Measurement of Intrathecally Synthesized Proteins in Mice
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Published on: November 29, 2019

Area of Science:

  • Immunology
  • Clinical Chemistry

Background:

  • Immunoglobulins (Ig) are protein molecules with antibody activity, produced by plasma cells.
  • There are five classes: IgG, IgA, IgM, IgD, and IgE, with IgG, IgA, and IgM being the most abundant in normal serum.

Purpose of the Study:

  • To explain the utility of quantitative serum immunoglobulin tests.
  • To highlight their role in diagnosing conditions associated with abnormal immunoglobulin levels.

Main Methods:

  • Quantitative analysis of serum immunoglobulin levels.
  • Focus on the three major classes: IgG, IgA, and IgM.

Main Results:

  • Serum immunoglobulin tests detect abnormal levels (excess or deficiency) of IgG, IgA, and IgM.
  • These variations are indicative of specific diseases and conditions.

Conclusions:

  • Quantitative serum immunoglobulin testing is crucial for diagnosing a range of diseases.
  • Monitoring IgG, IgA, and IgM levels provides valuable diagnostic information.