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Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...

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Related Experiment Video

Updated: May 12, 2026

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells
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Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells

Published on: September 1, 2019

A daunting task: manipulating leukocyte function with RNAi.

Dan Peer1

  • 1Laboratory of NanoMedicine, Department of Cell Research and Immunology, George S. Wise Faculty of Life Science, Tel Aviv, Israel. peer@tauex.tau.ac.il

Immunological Reviews
|April 5, 2013
PubMed
Summary
This summary is machine-generated.

Delivering RNA interference (RNAi) to leukocytes is difficult but crucial for treating blood cancers and infections. This review explores RNAi delivery strategies for these challenging immune cells.

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Last Updated: May 12, 2026

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Immunology

Background:

  • RNA interference (RNAi) has progressed into clinical applications.
  • Systemic RNAi delivery is established for liver and solid tumors.
  • Leukocyte delivery of RNAi remains a significant challenge.

Purpose of the Study:

  • To review progress in RNAi delivery strategies for leukocytes.
  • To highlight challenges and opportunities in manipulating leukocyte function via RNAi.
  • To discuss the potential of RNAi therapeutics for leukocyte-related diseases.

Main Methods:

  • Literature review of RNAi delivery strategies.
  • Analysis of challenges in transducing leukocytes with RNAi.
  • Exploration of opportunities for leukocyte manipulation.

Main Results:

  • Leukocyte delivery of RNAi is less advanced than for other tissues.
  • Specific delivery strategies are being developed for RNAi payloads to leukocytes.
  • Overcoming transduction barriers is key for therapeutic success.

Conclusions:

  • Effective RNAi delivery to leukocytes is critical for advancing therapies.
  • Targeting leukocyte function with RNAi offers promise for various diseases.
  • Further research into delivery methods is essential for clinical translation.