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Related Experiment Video

Updated: May 12, 2026

High-resolution Functional Magnetic Resonance Imaging Methods for Human Midbrain
10:06

High-resolution Functional Magnetic Resonance Imaging Methods for Human Midbrain

Published on: May 10, 2012

Mapping glutamate in subcortical brain structures using high-resolution GluCEST MRI.

Kejia Cai1, Anup Singh, David R Roalf

  • 1University of Pennsylvania, Radiology, Philadelphia, PA, USA.

NMR in Biomedicine
|April 5, 2013
PubMed
Summary

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This study introduces glutamate chemical exchange saturation transfer (GluCEST) MRI for mapping brain glutamate in subcortical regions. GluCEST imaging reveals glutamate distribution, aiding in understanding brain function and disorders.

Area of Science:

  • Neuroimaging
  • Neuroscience
  • Magnetic Resonance Imaging (MRI)

Background:

  • Accurate in vivo measurement of glutamate (Glu) in subcortical brain regions is crucial for understanding cognition and neuropsychiatric disorders.
  • Quantifying subcortical Glu presents significant challenges for existing neuroimaging techniques.

Purpose of the Study:

  • To develop and validate a novel MRI method, glutamate chemical exchange saturation transfer (GluCEST), for mapping Glu distributions in human subcortical brain structures.
  • To assess the sensitivity and reliability of GluCEST for in vivo Glu detection in millimolar concentrations.

Main Methods:

  • Utilized GluCEST MRI to map Glu distributions in human subcortical structures, including the amygdala and hippocampus.
  • Quantified GluCEST signal differences between gray matter and white matter.
Keywords:
amygdalachemical exchange saturation transfer (CEST)glutamatemagnetic resonance spectroscopy (MRS)subcortical brain structures

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Last Updated: May 12, 2026

High-resolution Functional Magnetic Resonance Imaging Methods for Human Midbrain
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High-resolution Functional Magnetic Resonance Imaging Methods for Human Midbrain

Published on: May 10, 2012

Single Synapse Indicators of Glutamate Release and Uptake in Acute Brain Slices from Normal and Huntington Mice
08:27

Single Synapse Indicators of Glutamate Release and Uptake in Acute Brain Slices from Normal and Huntington Mice

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  • Evaluated in vivo GluCEST detection sensitivity using MR spectroscopic data.

    • Main Results:

    • GluCEST signal was approximately 40% higher in gray matter compared to white matter.
    • The amygdala exhibited the highest GluCEST contrast among subcortical gray matter regions.
    • In vivo GluCEST detection sensitivity in subcortical gray matter was found to be approximately 0.8% mM(-1), consistent with prior reports.
    • GluCEST maps generally correlated with known Glu receptor distributions from PET studies.

    Conclusions:

    • High-resolution GluCEST MRI is a promising tool for mapping glutamate in subcortical brain structures.
    • This technique may aid in the diagnosis of brain disorders and monitoring treatment responses.
    • GluCEST imaging offers a novel approach to investigate the role of subcortical glutamate in neurological and psychiatric conditions.