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Related Concept Videos

Genomic DNA in Eukaryotes00:58

Genomic DNA in Eukaryotes

Eukaryotes have large genomes compared to prokaryotes. To fit their genomes into a cell, eukaryotic DNA is packaged extraordinarily tightly inside the nucleus. To achieve this, DNA is tightly wound around proteins called histones, which are packaged into nucleosomes that are joined by linker DNA and coil into chromatin fibers. Additional fibrous proteins further compact the chromatin, which is recognizable as chromosomes during certain phases of cell division.
Genomic DNA in Prokaryotes00:46

Genomic DNA in Prokaryotes

The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

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Related Experiment Video

Updated: May 12, 2026

Human Blastocyst Biopsy and Vitrification
10:59

Human Blastocyst Biopsy and Vitrification

Published on: July 26, 2019

Genomic DNA in human blastocoele fluid.

S Palini1, L Galluzzi, S De Stefani

  • 1IVF Unit, Cervesi' Hospital Cattolica, 47841 Cattolica (Rn), Italy. simonepalini@yahoo.it

Reproductive Biomedicine Online
|April 6, 2013
PubMed
Summary
This summary is machine-generated.

Genomic DNA is present in blastocoele fluid from embryos undergoing vitrification. This finding allows for potential embryo sex determination and genetic testing, aiding in reproductive health decisions.

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Area of Science:

  • Reproductive Medicine
  • Genetics
  • Embryology

Background:

  • Embryo cryopreservation via vitrification is standard in IVF.
  • Embryo collapse during vitrification involves blastocoele fluid withdrawal.
  • Blastocoele fluid metabolomics are studied for implantation efficiency prediction.

Purpose of the Study:

  • To investigate the presence of embryonic genomic DNA in blastocoele fluid.
  • To assess the feasibility of determining embryo sex from blastocoele fluid.
  • To explore the potential for comprehensive genetic analysis of blastocoele fluid.

Main Methods:

  • Real-time PCR was used to detect genomic DNA in blastocoele fluid.
  • Amplification of Y-chromosome (TSPY1) and autosomal (TBC1D3) genes for sex determination.
  • Whole-genome amplification and microarray comparative genomic hybridization were explored.

Main Results:

  • Genomic DNA was detected in approximately 90% of blastocoele fluid samples.
  • Embryo sex determination from blastocoele fluid was demonstrated.
  • Whole-genome amplification and preliminary aneuploidy detection were feasible.

Conclusions:

  • Blastocoele fluid is a source of embryonic DNA, enabling non-invasive sex determination.
  • This technique could facilitate genetic screening for X-linked disorders.
  • Further research is required to validate diagnostic accuracy for clinical application.