Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Other Glycolytic Pathways

Other Glycolytic Pathways

The pentose phosphate pathway (PPP) operates in parallel with glycolysis, facilitating the metabolism of both pentoses and glucose. This pathway consists of two distinct phases: the oxidative and non-oxidative phases. While it does not directly generate ATP, the intermediates formed during the process can integrate into glycolysis, contributing to cellular energy metabolism when required.Oxidative Phase: NADPH ProductionThe oxidative phase of the pentose phosphate pathway is primarily...

Amino Acid Biosynthetic Pathways

Amino Acid Biosynthetic Pathways

Amino acid biosynthesis is essential for cell growth, protein synthesis, and metabolic regulation. Cells generate essential and non-essential amino acids from metabolic intermediates to sustain vital biological functions. These intermediates originate from key metabolic pathways: glycolysis, the tricarboxylic acid (TCA) cycle, and the pentose phosphate pathway. Important precursors include α-ketoglutarate, pyruvate, oxaloacetate, phosphoenolpyruvate, and erythrose-4-phosphate, which provide...

Upstream Processing

Upstream Processing

Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...

Protein Networks

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...

Structure-Activity Relationships and Drug Design

Structure-Activity Relationships and Drug Design

Drug design is a dynamic field that involves discovering and developing new medications based on specific biological targets. This process heavily relies on structure-activity relationships (SAR) and quantitative structure-activity relationships (QSAR) to guide the design and optimization of efficient drugs.
SAR studies the intricate relationship between a drug's chemical structure and biological activity. It focuses on understanding how modifications to a drug's structure can influence its...

Bioreactor Controls-III

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Pathological diagnosis, differential diagnosis and origin investigation of easily misdiagnosed adult gastric duplication cysts.

Histology and histopathology·2021

Same author

3D V<sub>2</sub>O<sub>5</sub>-MoS<sub>2</sub>/rGO nanocomposites with enhanced peroxidase mimicking activity for sensitive colorimetric determination of H<sub>2</sub>O<sub>2</sub> and glucose.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2021

Same author

Abdominal computed tomography localizer image generation: A deep learning approach.

Computer methods and programs in biomedicine·2021

Same author

UNFERTILIZED EMBRYO SAC 12 phosphorylation plays a crucial role in conferring salt tolerance.

Plant physiology·2021

Same author

CUT&RUN identifies centromeric DNA regions of Rhodotorula toruloides IFO0880.

FEMS yeast research·2021

Same author

The Naturally Evolved <i>EPSPS</i> From Goosegrass Confers High Glyphosate Resistance to Rice.

Frontiers in plant science·2021

Search research articles

Related Experiment Video

Updated: May 12, 2026

A Web Tool for Generating High Quality Machine-readable Biological Pathways

A Web Tool for Generating High Quality Machine-readable Biological Pathways

Published on: February 8, 2017

Protein design for pathway engineering.

Dawn T Eriksen¹, Jiazhang Lian¹, Huimin Zhao²

¹Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States.

Journal of Structural Biology

|April 6, 2013

Summary

This summary is machine-generated.

Protein design is crucial for optimizing complex biochemical pathways in metabolic engineering. Engineering enzymes enhances substrate specificity, activity, and localization, leading to higher yields of desired compounds.

Keywords:

Biosensors Directed evolution Pathway engineering Protein engineering Rational design Synthetic biology

More Related Videos

Efficient Sampling of Genetically Encoded Biosensor Design Space Enabled with a Design of Experiments and Automation Workflow

Efficient Sampling of Genetically Encoded Biosensor Design Space Enabled with a Design of Experiments and Automation Workflow

Published on: October 17, 2025

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

Related Experiment Videos

Last Updated: May 12, 2026

A Web Tool for Generating High Quality Machine-readable Biological Pathways

A Web Tool for Generating High Quality Machine-readable Biological Pathways

Published on: February 8, 2017

Efficient Sampling of Genetically Encoded Biosensor Design Space Enabled with a Design of Experiments and Automation Workflow

Efficient Sampling of Genetically Encoded Biosensor Design Space Enabled with a Design of Experiments and Automation Workflow

Published on: October 17, 2025

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

Area of Science:

Biochemistry
Metabolic Engineering
Synthetic Biology

Background:

Biosynthetic production of complex compounds involves multi-enzyme pathways, which present coordination challenges.
While metabolic engineering tools optimize pathway flux, individual enzyme characteristics can limit productivity.
Protein design is essential for overcoming these limitations in pathway engineering.

Purpose of the Study:

To review strategies for applying protein design to optimize flux in engineered biochemical pathways.
To highlight how protein engineering improves the efficiency and productivity of biosynthetic pathways.
To showcase the role of protein design in producing novel compounds.

Main Methods:

Engineering proteins for altered substrate specificity and selectivity.
Improving enzyme catalytic activity through protein modification.
Reducing mass transfer limitations via targeted protein localization.
Minimizing substrate/product inhibition through protein design.
Developing biosensors for high-throughput screening and cell signaling customization.

Main Results:

Successfully engineered pathways demonstrate significantly increased productivity.
Protein design enables the production of novel compounds through optimized pathways.
Strategies address limitations posed by enzyme characteristics and pathway coordination.

Conclusions:

Judicious protein design is critical for advancing metabolic and pathway engineering.
Protein engineering offers versatile strategies to enhance biosynthetically produced compound yields.
Optimized pathways through protein design are key to efficient bioproduction and novel molecule discovery.