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Related Concept Videos

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Super-resolution Fluorescence Microscopy

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Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
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A localized surface plasmon resonance imaging instrument for multiplexed biosensing.

Julia A Ruemmele1, W Paige Hall, Laura K Ruvuna

  • 1Department of Chemistry, Northwestern University, Evanston, Illinois 60208-3133, United States.

Analytical Chemistry
|April 9, 2013
PubMed
Summary

This study introduces macroscale nanoparticle arrays for multiplexed localized surface plasmon resonance imaging (LSPRi). This advancement enables high-throughput, label-free analysis of molecular binding interactions with enhanced sensitivity and selectivity.

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A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Area of Science:

  • Nanotechnology
  • Spectroscopy
  • Biophysics

Background:

  • Localized surface plasmon resonance (LSPR) spectroscopy offers sensitive, label-free surface interaction analysis.
  • LSPR imaging (LSPRi) enhances throughput by probing multiple sensors simultaneously.
  • Previous LSPRi focused on small-scale, single-particle sensing.

Purpose of the Study:

  • To develop macroscale nanoparticle arrays for multiplexed LSPRi compatible with common patterning techniques.
  • To demonstrate high-throughput, multiplexed LSPR imaging for molecular binding analysis.
  • To showcase the selectivity and sensitivity of the new LSPR sensing platform.

Main Methods:

  • Fabrication of macroscale nanoparticle arrays using dip-pen nanolithography and microfluidic devices.
  • Implementation of multiplexed LSPR imaging to analyze molecular binding kinetics.
  • Demonstration of DNA hybridization for assessing sensor selectivity.

Main Results:

  • Successful creation of defined macroscale nanoparticle arrays for LSPRi.
  • First demonstration of multiplexed LSPR imaging enabling complete Langmuir binding curve collection on a single sensor.
  • High selectivity shown through specific DNA sequence hybridization.
  • Uniform sensitivity and tailorable optical properties of the LSPR arrays.

Conclusions:

  • Macroscale nanoparticle arrays significantly advance multiplexed LSPR imaging capabilities.
  • The developed platform provides a robust solution for high-throughput, label-free molecular interaction analysis.
  • This technology is ideal for diverse applications requiring sensitive and selective biomolecular detection.