Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reverse Janssen effect with non-spherical grains.

Physical review. E·2026
Same author

Liquidlike Dynamics in Ordered Soft-Particle Systems.

Physical review letters·2026
Same author

Multi-media occurrence, bioaccumulation, and exposure assessment of PFAS across a Midwestern U.S. State.

Environmental research·2026
Same author

Structure-dynamics decoupling in soft-colloid suspensions.

Nature communications·2025
Same author

Generating and leveraging explanations of AI/ML models in materials and manufacturing research.

Patterns (New York, N.Y.)·2025
Same author

Hidden order in active nematic defects.

Proceedings of the National Academy of Sciences of the United States of America·2025

Related Experiment Video

Updated: May 12, 2026

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches
10:07

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

Published on: October 8, 2021

Altering colloidal surface functionalization using DNA encapsulated inside monodisperse gelatin microsphere

James O Hardin1, Alberto Fernandez-Nieves, Carlos J Martinez

  • 1School of Materials Science and Engineering, Georgia Institute of Technology, Atlanta, Georgia 30032-0245, United States.

Langmuir : the ACS Journal of Surfaces and Colloids
|April 9, 2013
PubMed
Summary
This summary is machine-generated.

This study introduces a novel method for DNA hybridization using temperature-triggered release of encapsulated DNA targets from colloidal assemblies. This approach enhances surface hybridization efficiency by releasing secondary DNA targets at 37 °C.

More Related Videos

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
11:40

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization
06:26

Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization

Published on: January 24, 2025

Related Experiment Videos

Last Updated: May 12, 2026

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches
10:07

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

Published on: October 8, 2021

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
11:40

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization
06:26

Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization

Published on: January 24, 2025

Area of Science:

  • Biotechnology
  • Materials Science
  • Molecular Biology

Background:

  • Traditional DNA hybridization relies on introducing soluble oligonucleotides to bulk solution.
  • This method can be inefficient and difficult to control for surface-based applications.

Purpose of the Study:

  • To develop an alternative method for controlled DNA hybridization on surfaces.
  • To investigate the use of encapsulated DNA targets released via temperature stimulus.

Main Methods:

  • Fabrication of uniform gelatin microspheres using microfluidics for DNA encapsulation.
  • Loading microspheres with secondary DNA targets and capping with a polyelectrolyte bilayer.
  • Coating polystyrene microspheres with DNA probes and hybridization partners, followed by assembly with gelatin microspheres.

Main Results:

  • Successful encapsulation and temperature-triggered release of secondary DNA targets from gelatin microspheres at 37 °C.
  • Released secondary DNA targets competitively displaced shorter hybridization partners on polystyrene surfaces.
  • Demonstrated a novel approach for controlled surface hybridization activity.

Conclusions:

  • Encapsulating DNA targets within colloidal assemblies offers a controlled method for surface hybridization.
  • Temperature-triggered release provides a precise mechanism to initiate hybridization events.
  • This technique has potential applications in biosensing and DNA nanotechnology.