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Related Concept Videos

Optimizing Chromatographic Separations01:15

Optimizing Chromatographic Separations

Optimizing chromatographic separations is crucial for obtaining clean separations in a minimum amount of time. Optimization is required for several factors, including kinetic effects related to band broadening, plate height, capacity factor, and separation factor.
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
Principles Of Column Chromatography01:13

Principles Of Column Chromatography

The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
Chromatography: Introduction01:10

Chromatography: Introduction

Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
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High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
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Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
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Published on: September 2, 2020

Tutorial: simulating chromatography with Microsoft Excel Macros.

Akinde Kadjo1, Purnendu K Dasgupta1

  • 1Department of Chemistry and Biochemistry, The University of Texas at Arlington, Arlington, TX 76019-0065, USA.

Analytica Chimica Acta
|April 9, 2013
PubMed
Summary

This study demonstrates how Microsoft Excel macros can simulate chromatography, offering a transparent and accessible method for understanding complex separation processes. This approach aids future chromatographers in grasping fundamental principles and exploring various chromatographic scenarios.

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Area of Science:

  • Analytical Chemistry
  • Computational Chemistry

Background:

  • Chromatography is a fundamental technique in analytical chemistry.
  • Existing simulation software often lacks transparency for beginners.
  • Microsoft Excel is widely accessible and its functionality is well understood.

Purpose of the Study:

  • To develop a transparent and accessible method for simulating chromatographic separations using Microsoft Excel.
  • To provide a tool for novice chromatographers to intuitively understand chromatographic principles.
  • To enable the simulation of various chromatographic conditions and complex scenarios.

Main Methods:

  • Utilizing spreadsheet rows to represent plates and automated subroutines (Macros) for mobile phase movement.
  • Simulating isocratic, gradient elution, nonlinear isotherms, column overloading, and analyte degradation.
  • Employing Excel's color gradation feature for real-time visualization of analyte band migration.

Main Results:

  • Successfully simulated various chromatographic conditions, including gradients and complex scenarios not easily modeled by other software.
  • Provided real-time views of separation development and end-column detection.
  • Enabled easy modification of parameters to examine their effects on separations.

Conclusions:

  • Spreadsheet-based simulation offers a transparent and versatile platform for learning and exploring chromatography.
  • This method enhances understanding of chromatographic processes for students and researchers.
  • The accessibility of Excel makes this simulation technique widely applicable.