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A Simple Microaspiration Technique for Isolating Somatic Cells from Cryopreserved Equine Semen as Nuclear Donors for Cloning
Published on: December 19, 2025
New seminal plasma removal method for freezing stallion semen
C Ramires Neto1, G A Monteiro, R F Soares
1College of Veterinary Medicine and Animal Science, Univ Estadual Paulista, Department of Animal Reproduction and Veterinary Radiology, Botucatu, Sao Paulo, Brazil.
Theriogenology
|April 9, 2013
Summary
A new Sperm Filter effectively removes seminal plasma from stallion semen before cryopreservation, matching centrifugation
Area of Science:
- Veterinary Reproduction
- Sperm Biology
- Cryopreservation Technology
Background:
- Seminal plasma removal is crucial for equine semen cryopreservation.
- Centrifugation, the standard method, may damage sperm.
- A novel Sperm Filter offers a potential alternative.
Purpose of the Study:
- To evaluate the efficacy of a new Sperm Filter for seminal plasma removal in stallion semen.
- To compare the Sperm Filter method with traditional centrifugation before cryopreservation.
Main Methods:
- Semen from 31 stallions was divided into two groups for cryopreservation.
- Group 1: Seminal plasma removed using the Sperm Filter.
- Group 2: Seminal plasma removed by centrifugation (600×g for 10 minutes).
Main Results:
- No significant differences in sperm kinetics or plasma membrane integrity between groups.
- Sperm recovery rate was significantly higher in the Sperm Filter group (89.4%) compared to centrifugation (80.9%).
- Filtering proved more practical with fewer sperm losses.
Conclusions:
- The Sperm Filter is as effective as centrifugation for seminal plasma removal in stallions.
- The Sperm Filter offers a more practical and efficient alternative, reducing sperm loss during cryopreservation preparation.
