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Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
Published on: December 15, 2017
Protein engineering of enzymes for process applications
1Center for Process Engineering and Technology, Department of Chemical and Biochemical Engineering, Technical University of Denmark, DK-2800 Lyngby, Denmark. jw@kt.dtu.dk
Current Opinion in Chemical Biology
|April 9, 2013
Summary
Enzyme modification is advancing for industrial use, shifting focus from substrate scope to process requirements like thermodynamics and stability. Future work will integrate biocatalysis with chemical steps.
Area of Science:
- Biocatalysis and Enzyme Engineering
- Industrial Biotechnology
Background:
- Enzyme modification techniques have significantly advanced, enabling tailored biocatalysts for industrial applications.
- Previous research primarily focused on expanding substrate range and improving selectivity of enzymes.
- Emerging opportunities in enzyme modification are increasingly directed towards enabling robust process applications.
Purpose of the Study:
- To discuss the challenges associated with enzyme modification for industrial process requirements.
- To highlight key parameters including reaction thermodynamics, specific activity, kinetics, and stability.
- To explore future research directions for enzyme modification in industrial contexts.
Main Methods:
- Literature review and synthesis of current challenges in enzyme modification for process applications.
- Analysis of critical factors influencing enzyme performance under industrial conditions.
- Discussion of integration strategies for biocatalysis with chemical processes.
Main Results:
- Enzyme modification for industrial processes faces challenges in meeting thermodynamic, kinetic, and stability requirements.
- Achieving specific activity under required process conditions is a key hurdle.
- The need for robust enzyme stability under operational parameters is paramount.
Conclusions:
- Successful industrial biocatalysis requires enzyme modifications that address process-specific demands beyond substrate scope and selectivity.
- Future research should focus on optimizing enzymes for thermodynamic efficiency, kinetics, and stability.
- Integrating biocatalysis with adjacent chemical transformations represents a promising avenue for process intensification.
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