Related Experiment Video
Updated: Jan 20, 2026

Quantitative, Real-time Analysis of Base Excision Repair Activity in Cell Lysates Utilizing Lesion-specific Molecular Beacons
Published on: August 6, 2012
Assistant DNA recycling with nicking endonuclease and molecular beacon for signal amplification using a
Fenglei Gao1, Jianping Lei, Huangxian Ju
1State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, PR China.
Abstract:
A simple and universal method for ultrasensitive "signal on" detection of DNA was developed with a target-complementary arched structure to release assistant DNA, which was recycled with nicking endonuclease to amplify the detectable fluorescent signal of molecular beacons.
Related Concept Videos
10:22Designed for Molecular Recycling: A Lignin-Derived Semi-aromatic Biobased Polymer
09:31Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites
12:20Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
15:01Quantitative, Real-time Analysis of Base Excision Repair Activity in Cell Lysates Utilizing Lesion-specific Molecular Beacons
09:36Visualization of Mitochondrial DNA Replication in Individual Cells by EdU Signal Amplification
Complementary DNA
