Related Experiment Video
Updated: May 12, 2026
Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
Published on: October 12, 2017
Improvement of the lipophilic-oxygen radical absorbance capacity (L-ORAC) method and single-laboratory validation
Jun Watanabe1, Tomoyuki Oki, Jun Takebayashi
1National Food Research Institute, National Agriculture and Food Research Organization, Ibaraki, Japan. nabej@affrc.go.jp
Abstract:
We improved the procedure for lipophilic-oxygen radical absorbance capacity (L-ORAC) measurement for better repeatability and intermediate precision. A sealing film was placed on the assay plate, and glass vials and microdispensers equipped with glass capillaries were used. The antioxidant capacities of food extracts can be evaluated by this method with nearly the same precision as antioxidant solutions.
