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Harvesting and Cryo-cooling Crystals of Membrane Proteins Grown in Lipidic Mesophases for Structure Determination by Macromolecular Crystallography
18:45

Harvesting and Cryo-cooling Crystals of Membrane Proteins Grown in Lipidic Mesophases for Structure Determination by Macromolecular Crystallography

Published on: September 2, 2012

Conformational transitions at an S-layer growing boundary resolved by cryo-TEM

Luis R Comolli1, Cristina E Siegerist, Seong-Ho Shin

  • 1Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. lrcomolli@lbl.gov

Angewandte Chemie (International Ed. in English)
|April 9, 2013
PubMed
Summary

No abstract available in PubMed .

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Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

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