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DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

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Related Experiment Video

Updated: May 12, 2026

Collection and Extraction of Saliva DNA for Next Generation Sequencing

Collection and Extraction of Saliva DNA for Next Generation Sequencing

Published on: August 27, 2014

High-yield RNA-extraction method for saliva.

Pratibala Pandit¹, Justin Cooper-White, Chamindie Punyadeera

¹Australian Institute for Bioengineering and Nanotechnology, University of Queensland, St. Lucia, Queensland, Australia.

Clinical Chemistry

|April 9, 2013

Summary

This summary is machine-generated.

A new, cost-effective method using QIAzol lysis reagent efficiently isolates high yields of total RNA from saliva for head and neck cancer transcriptomic studies.

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Area of Science:

Molecular Biology
Biochemistry
Oncology

Background:

Salivary diagnostics offer a noninvasive approach for disease detection.
Saliva is a valuable biofluid for identifying RNA targets in head and neck cancer.
Current methods for RNA isolation from saliva need improvement in yield and cost-effectiveness.

Purpose of the Study:

To develop a robust, simple, and cost-effective method for isolating high yields of total RNA from saliva.
To enable downstream expression studies using RNA extracted from saliva.
To facilitate the translation of saliva transcriptomic studies into clinical settings.

Main Methods:

Collected oral whole saliva from healthy controls and head and neck cancer patients.
Utilized QIAzol lysis reagent for RNA extraction from cell-free saliva and cell pellets.
Performed cDNA synthesis and real-time PCR for target gene expression analysis.

Main Results:

The in-house QIAzol method yielded significantly higher RNA quantities (0.89-7.1 μg) compared to a commercial kit.
Isolated RNA maintained good purity, with A260/A280 ratios between 1.6 and 1.9.
Successfully extracted RNA from saliva samples archived for over two years without RNase inhibitors.

Conclusions:

The developed in-house QIAzol method is robust, simple, and provides high RNA yields from saliva.
This method is suitable for saliva transcriptomic studies.
The technique can be implemented for clinical applications.