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Regenerative electronic biosensors using supramolecular approaches.

Xuexin Duan1, Nitin K Rajan, David A Routenberg

  • 1Department of Electrical Engineering, Yale University, New Haven, Connecticut 06520, United States.

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This study introduces reusable Si nanowire FET biosensors using β-cyclodextrin (β-CD) and supramolecular linkers. The regenerative sensors enable precise detection and quantification of chiral molecules, enhancing biosensing capabilities.

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Area of Science:

  • Materials Science
  • Nanotechnology
  • Biochemistry

Background:

  • Development of regenerative biosensors is crucial for sustainable and cost-effective diagnostics.
  • Si nanowire field-effect transistors (Si-NW FETs) offer high sensitivity for biosensing applications.
  • Functionalization of Si-NWs with supramolecular interfaces enables adaptable sensor design.

Purpose of the Study:

  • To develop a regenerative supramolecular interface for Si-NW FET biosensors.
  • To demonstrate the selective removal and regeneration of the sensor surface.
  • To explore the potential for stereoselective sensing using chiral recognition.

Main Methods:

  • Functionalization of Si-NWs with β-cyclodextrin (β-CD) and orthogonal supramolecular linkers.
  • Utilizing the streptavidin (SAv)-biotin system for high-affinity binding and subsequent competitive desorption.
  • Employing concentrated β-CD for selective removal of bound SAv and linkers to regenerate the sensor surface.
  • Quantifying enantiomeric composition of chiral targets to demonstrate stereoselectivity.

Main Results:

  • Successful demonstration of a fully recyclable Si-NW FET biosensor using the SAv-biotin system.
  • Selective removal of bound streptavidin and linkers achieved through competitive desorption with β-CD.
  • Regeneration of the sensor surface confirmed for repeated use.
  • Quantification of enantiomeric composition of chiral targets, showcasing stereoselective sensing capabilities.

Conclusions:

  • A novel supramolecular interface enables regenerative Si-NW FET biosensors.
  • The developed system allows for efficient sensor regeneration and reuse.
  • The β-CD functionalization provides a platform for stereoselective biosensing, enabling chiral analysis.