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Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells
Published on: October 13, 2023
High throughput capture of circulating tumor cells using an integrated microfluidic system.
Zongbin Liu1, Wang Zhang, Fei Huang
1Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, PR China.
Biosensors & Bioelectronics
|April 10, 2013
Summary
This study presents an integrated microfluidic system for efficient circulating tumor cell (CTC) isolation. The device achieves high purity and throughput for cancer cell capture from blood samples, aiding cancer research.
Area of Science:
- Biomedical Engineering
- Oncology
- Microfluidics
Background:
- Circulating tumor cells (CTCs) are crucial biomarkers for cancer diagnosis, prognosis, and treatment monitoring.
- Efficient and high-purity isolation of CTCs from blood remains a significant challenge in clinical applications.
Purpose of the Study:
- To develop and validate an integrated microfluidic system for rapid and efficient isolation and capture of circulating tumor cells.
- To enhance cancer cell enrichment, throughput, and purity for downstream analysis.
Main Methods:
- Integration of microfluidic deterministic lateral displacement array with affinity-based cell capture architecture.
- Utilized breast cancer cells spiked in blood samples for performance evaluation.
- Quantified enrichment factor, processing throughput, capture yield, and capture purity.
Main Results:
- Achieved an enrichment factor of 1500× for breast cancer cells.
- Demonstrated a high processing throughput of 9.6 mL/min.
- Obtained 90% capture yield and over 50% capture purity at a cell concentration of 10^2 cells/mL.
Conclusions:
- The integrated microfluidic platform enables high-efficiency CTC capture with excellent recovery rates, purity, and stability.
- This system shows significant potential for clinical applications, including cancer cell culture and drug screening.
