Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Peanut alkaline lipase.

T H Sanders, H E Pattee

    Lipids
    |January 1, 1975
    PubMed
    Summary
    This summary is machine-generated.

    Researchers isolated peanut alkaline lipase (glycerol ester hydrolase EC 3.1.1.3) from peanut seeds. Enzyme activity increased during development, with a determined Km of 2.6 x 10-4M and molecular weight of 55,000.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Characterization of alkylmethoxypyrazines contributing to earthy/bell pepper flavor in farmstead cheddar cheese.

    Journal of food science·2008
    Same author

    The effect of refrigerated and frozen storage on butter flavor and texture.

    Journal of dairy science·2008
    Same author

    The effect of transglutaminase crosslinking on the rheological characteristics of heated peanut flour dispersions.

    Journal of food science·2007
    Same author

    Transglutaminase polymerization of peanut proteins.

    Journal of agricultural and food chemistry·2007
    Same author

    Non-detectable levels of trans-fatty acids in peanut butter.

    Journal of agricultural and food chemistry·2001
    Same author

    Sensory attribute variation in low-temperature-stored roasted peanut paste.

    Journal of agricultural and food chemistry·2000
    Same journal

    Oral Delivery of Tocotrienols: Addressing Pharmacokinetic Challenges With Formulation-Based Strategies.

    Lipids·2026
    Same journal

    Very Low Cholesterol Non-Cardiovascular Risks: A Critical Appraisal and Framework for Individualized Management.

    Lipids·2026
    Same journal

    Exometabolomics Reveals Metabolic Modulation Induced by Chia Oil Supplementation in Adipose Tissue Explants From Obese Mice.

    Lipids·2026
    Same journal

    Analysis of the Value of Combined Detection of Serum miR-302a-3p and Low Density Lipoprotein Cholesterol in the Diagnosis of Coronary Heart Disease.

    Lipids·2026
    Same journal

    Association Between C-Reactive Protein-Triglyceride Glucose Index, and the Risk of Osteoarthritis and Rheumatoid Arthritis: Results From NHANES 1999-2010.

    Lipids·2026
    Same journal

    Transcriptomic Analysis of Shared Lipid Metabolism in Chronic Hepatitis B and Non-Alcoholic Fatty Liver Disease Using Weighted Gene Expression Network Analysis.

    Lipids·2026
    See all related articles

    Area of Science:

    • Biochemistry
    • Plant Science

    Background:

    • Lipases are crucial enzymes in lipid metabolism.
    • Peanut seeds are a significant source of plant-based oils and proteins.

    Purpose of the Study:

    • To isolate and characterize peanut alkaline lipase (glycerol ester hydrolase EC 3.1.1.3).
    • To understand the enzyme's properties during peanut seed development.

    Main Methods:

    • Isolation of lipase from developing and germinated peanut seeds (Arachis hypogaea L. var. NC-2) using acetone powders.
    • Enzyme activity assays to determine pH optimum and reaction kinetics.
    • Molecular weight estimation using Sephadex gel filtration and SDS-gel electrophoresis.

    Main Results:

    • Peanut alkaline lipase exhibited optimal activity at pH 8.5.

    Related Experiment Videos

  • Enzyme activity per seed increased with developmental stage.
  • The reaction rate was linear with enzyme concentration and time (>60 min).
  • The Michaelis constant (Km) was determined to be 2.6 x 10-4M.
  • The estimated molecular weight was approximately 55,000 Da.

    • Conclusions:

    • Peanut alkaline lipase is present in developing and germinating seeds.
    • Enzyme levels increase during seed maturation.
    • Characterization provides foundational data for potential biotechnological applications.