Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cross-domain transfer learning strategy enhances interpretability of deep learning model explanations.

Scientific reports·2026
Same author

Estimating changes in systolic blood pressure based on pulse wave morphology using paired segment comparison.

Physiological measurement·2026
Same author

Outcomes of a Foundational Sciences Daily Engagement Game in Medical Education.

Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology·2026
Same author

Low-Background Cardiac Troponin I Detection via In Situ Self-Color-Changing Lateral Flow Immunoassay Enabled by H<sub>2</sub>O<sub>2</sub>-Triggered Cerium Oxide Nanoprobes.

Analytical chemistry·2026
Same author

Vancomycin: a dura damaging friend of the spine surgeon? An experimental study.

European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·2026
Same author

Digital PCR as a potential reference measurement procedure to support monkeypox virus/Orthopoxvirus external quality assessment schemes.

Methods (San Diego, Calif.)·2026
Same journal

RETRACTED: Zhang et al. A Novel Framework for Reconstruction and Imaging of Target Scattering Centers via Wide-Angle Incidence in Radar Networks. <i>Sensors</i> 2025, <i>25</i>, 6802.

Sensors (Basel, Switzerland)·2026
Same journal

Enhancing Unsupervised Multi-Source Domain Adaptation for Person Re-Identification via Mixture of Experts and Graph-Based Relation.

Sensors (Basel, Switzerland)·2026
Same journal

Development of an Instrumented Glove for Palmar Pressure Assessment in Kayakers.

Sensors (Basel, Switzerland)·2026
Same journal

Development and Experimental Validation of an Autonomous IoT-Based Monitoring System for Real-Time Water Quality Assessment in the Amazon River.

Sensors (Basel, Switzerland)·2026
Same journal

Semi-Supervised Adversarial Learning Framework for Controller Area Network Bus Intrusion Detection.

Sensors (Basel, Switzerland)·2026
Same journal

Smart Optimization Method for Safety Signs in Innovative Manufacturing Environments Integrating Industrial Field IoT Sensors and Knowledge Graphs.

Sensors (Basel, Switzerland)·2026
See all related articles

Related Experiment Video

Updated: May 12, 2026

Microfluidic Approach to Resolve Simultaneous and Sequential Cytokine Secretion of Individual Polyfunctional Cells
09:43

Microfluidic Approach to Resolve Simultaneous and Sequential Cytokine Secretion of Individual Polyfunctional Cells

Published on: March 8, 2024

Microflow cytometers with integrated hydrodynamic focusing.

Marcin Frankowski1, Janko Theisen, Andreas Kummrow

  • 1Physikalisch-Technische Bundesanstalt, Abbestrasse 2-12, 10587 Berlin, Germany. marcin.frankowski@ptb.de

Sensors (Basel, Switzerland)
|April 11, 2013
PubMed
Summary
This summary is machine-generated.

This study shows microfluidic chips are effective for high-throughput blood cell analysis using hydrodynamic focusing. These devices enable precise cell positioning for accurate counting and differentiation, including T-lymphocytes.

More Related Videos

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)
07:19

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)

Published on: June 28, 2017

Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone
06:42

Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone

Published on: April 11, 2013

Related Experiment Videos

Last Updated: May 12, 2026

Microfluidic Approach to Resolve Simultaneous and Sequential Cytokine Secretion of Individual Polyfunctional Cells
09:43

Microfluidic Approach to Resolve Simultaneous and Sequential Cytokine Secretion of Individual Polyfunctional Cells

Published on: March 8, 2024

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)
07:19

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)

Published on: June 28, 2017

Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone
06:42

Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone

Published on: April 11, 2013

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Cell Biology

Background:

  • High-throughput analysis of blood cells is crucial for diagnostics.
  • Microfluidic devices offer potential for miniaturized and efficient cell analysis.
  • Hydrodynamic focusing is a key technique for precise particle manipulation in microchannels.

Purpose of the Study:

  • To evaluate the suitability of microfluidic structures for high-throughput blood cell analysis.
  • To compare two hydrodynamic focusing techniques: cascade focusing and spin focusing.
  • To demonstrate the application of microfluidic flow cytometry for T-lymphocyte detection.

Main Methods:

  • Development and implementation of microfluidic chips utilizing two-stage cascade and spin focusing principles.
  • Injection of micro-particle or blood cell suspensions into sheath fluid at high flow rates.
  • Evaluation of hydrodynamic particle positioning stability using fluorescence signal analysis and coefficient of variation.
  • Detection of immunologically labeled T-lymphocytes (CD3+, CD4+) in blood samples.

Main Results:

  • Achieved particle velocities suitable for high-throughput analysis (meters per second).
  • Demonstrated stable hydrodynamic particle positioning with a coefficient of variation of approximately 3% for cascade focusing.
  • Obtained similar performance with spin focusing at 1.5 times lower sample flow rates.
  • Successfully detected CD3 positive and CD4 positive T-lymphocytes, showcasing the micro flow cytometer's potential.

Conclusions:

  • Microfluidic devices employing hydrodynamic focusing are suitable for high-throughput blood cell analysis.
  • Both cascade and spin focusing methods provide stable and precise particle manipulation for cell differentiation and counting.
  • The developed micro flow cytometer demonstrates significant potential for clinical applications, including T-lymphocyte enumeration.