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Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...

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Related Experiment Video

Updated: May 12, 2026

Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions
10:44

Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions

Published on: October 21, 2016

Analysis of protein interactions using fluorescence technologies.

Yuling Yan1, Gerard Marriott

  • 1Department of Physiology, University of Wisconsin-Madison, 1300 University Ave, Madison, WI 53706, USA.

Drug Discovery Today
|April 12, 2013
PubMed
Summary
This summary is machine-generated.

Biophotonics and fluorescence methods analyze protein interactions in vitro. New technologies are needed for real-time, spatial analysis of protein complexes within cells for comprehensive proteome studies.

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Last Updated: May 12, 2026

Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions
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Published on: October 21, 2016

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11:46

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Published on: May 26, 2014

Imaging Protein-protein Interactions in vivo
11:15

Imaging Protein-protein Interactions in vivo

Published on: October 10, 2010

Area of Science:

  • Biophotonics
  • Proteomics
  • Cellular Biology

Background:

  • Biophotonics techniques, particularly fluorescence-based methods, are standard for in vitro protein interaction analysis in high-throughput proteomics.
  • Cellular imaging reveals protein activity is often controlled by transient, large protein complexes formed at specific intracellular locations.

Purpose of the Study:

  • To highlight the need for advanced technologies capable of analyzing protein interactions within living cells.
  • To emphasize the requirement for time- and spatially-resolved, multiplexed analysis for a complete understanding of the human proteome.

Main Methods:

  • Review of current biophotonics and fluorescence-based techniques in proteomics.
  • Discussion of limitations in analyzing intracellular protein interactions.
  • Conceptual outline of requirements for next-generation cellular analysis technologies.

Main Results:

  • Current in vitro methods do not fully capture the dynamic, spatial nature of protein interactions within cells.
  • Protein complex formation and regulation occur dynamically within specific cellular compartments.
  • A gap exists in technologies for comprehensive, in-cell proteome analysis.

Conclusions:

  • Systematic functional analysis of the human proteome necessitates technologies that bridge the gap between in vitro and in vivo studies.
  • Future research should focus on developing tools for time- and spatially-resolved, multiplexed analysis of intracellular protein interactions.