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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Protein-Protein Interfaces02:04

Protein-Protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...

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Related Experiment Video

Updated: May 12, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Interplay of transcriptomics and proteomics.

Priti S Hegde1, Ian R White, Christine Debouck

  • 1Department of Transcriptome Analysis, GlaxoSmithKline Pharmaceutical Research & Development, 1250 South Collegeville Road, Collegeville, PA 19426, USA.

Drug Discovery Today
|April 12, 2013
PubMed
Summary
This summary is machine-generated.

Global transcriptomics and proteomics offer powerful insights but face practical challenges. Careful study design is crucial for comparing these omic data types, with future work addressing splicing and modifications.

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Area of Science:

  • Molecular biology
  • Genomics
  • Proteomics

Background:

  • Global omics profiling, including transcriptomics and proteomics, is attractive for comprehensive biological analysis.
  • Significant practical and biological differences exist between transcriptomic and proteomic analyses.
  • Transcriptomics is a mature, high-throughput technology, while proteomics faces limitations in coverage and depth.

Purpose of the Study:

  • To highlight the differences and similarities between transcriptomic and proteomic profiling.
  • To discuss the challenges and considerations for comparing these two omics datasets.
  • To identify future research directions in integrated omics analysis.

Main Methods:

  • Comparative analysis of transcriptomic and proteomic methodologies.
  • Discussion of inherent biological variations affecting protein and mRNA analysis.
  • Review of strategies for data integration and interpretation.

Main Results:

  • Transcriptomics offers broad, cost-effective quantification of mRNA species.
  • Proteomics is constrained by protein abundance, stability, and post-translational modifications.
  • Successful comparison of transcriptomic and proteomic data requires careful experimental design.

Conclusions:

  • Despite current limitations, transcriptomic and proteomic data can be meaningfully compared.
  • Future research should focus on addressing challenges like differential splicing and post-translational modifications.
  • Integrated omics approaches hold promise for deeper biological understanding.