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Updated: May 12, 2026

Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin
Published on: January 17, 2012
[Identification of calcium/calmodulin-binding receptor-like kinase GsCBRLK-interactive proteins using yeast
Shan-Shan Yang1, Xiao-Li Sun, Yang Yu
1College of Life Sciences, Northeast Agriculture University, Harbin, China. yangshanshanhp@sina.com
Abstract:
GsCBRLK (calcium/calmodulin-binding receptor-like kinase from Glycine soja) links ABA (abscisic acid)- and salt-induced calcium/calmodulin signal in plant cells. In order to study the molecular mechanisms of GsCBLRK, the salt-treated Glycine soja cDNA library was screened with pBT3-STE-CBRLK as bait plasmid using yeast two hybrid system. Two positive clones (SNARE and 14-3-3 protein) were identified by constructing cDNA library of wild soybean under salt treatment, membrane system yeast two hybrid screening, multiple screen, rotary validation, bioinformatic analysis and interaction identification in yeast.
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