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Related Experiment Video

Updated: May 12, 2026

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling
07:18

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Published on: May 21, 2020

Comparative transcriptome profiling of developing caryopses from two rice cultivars with differential dormancy.

Sun Mi Huh1, Yong-sic Hwang, Young Seop Shin

  • 1Molecular Breeding Division, National Academy of Agricultural Science, RDA, Suwon 441-857, Republic of Korea.

Journal of Plant Physiology
|April 13, 2013
PubMed
Summary

Rice seed dormancy is crucial for quality and yield. This study found that differences in rice dormancy between cultivars Gopum and Samgwang are linked to gene expression during seed maturation, not abscisic acid levels.

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06:31

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis

Published on: January 11, 2017

Area of Science:

  • Plant Science
  • Molecular Biology
  • Agronomy

Background:

  • Pre-harvest sprouting (PHS) in rice leads to economic losses due to reduced grain quality and yield.
  • Both PHS and excessively deep dormancy negatively impact rice cultivation, highlighting the need for optimal seed dormancy.
  • Understanding the genetic basis of seed dormancy is critical for improving rice agronomic traits.

Purpose of the Study:

  • To investigate the transcriptomic differences associated with varying seed dormancy levels in two Korean rice cultivars.
  • To identify genes and pathways involved in the establishment of dormancy during caryopsis development.
  • To explore the role of abscisic acid (ABA) in differential dormancy between rice cultivars.

Main Methods:

  • Comparative transcriptomic analysis (RNA profiling) of two rice cultivars (Gopum and Samgwang) at different developmental stages (3-6, 25, and 40 days after heading).
  • K-means clustering to group genes with similar or distinct expression profiles.
  • Analysis of gene expression patterns related to seed maturation and dormancy.

Main Results:

  • Significant transcriptomic differentiation was observed at 25 days after heading (DAH), coinciding with the most prominent differences in dormancy.
  • Genes involved in seed maturation showed distinct differential expression profiles between the cultivars.
  • Differential gene expression correlated with the progression of caryopsis development, suggesting maturation influences dormancy establishment.
  • Gopum cultivar exhibited faster caryopsis development, potentially leading to earlier dormancy release compared to Samgwang.
  • No significant differences in RNA profiles related to abscisic acid (ABA) content or sensitivity were found, suggesting ABA is not the primary driver of observed dormancy differences.

Conclusions:

  • Seed dormancy in rice is strongly influenced by transcriptomic changes during caryopsis maturation.
  • The speed of caryopsis development appears to be a key factor differentiating dormancy levels between rice cultivars.
  • Abscisic acid (ABA) is unlikely to be directly involved in the observed differences in seed dormancy between Gopum and Samgwang rice cultivars.