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Updated: Jun 8, 2025

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries
Published on: August 12, 2019
On-DNA Mannich Reaction for DNA-Encoded Library Synthesis
Danila Ryzhikh1, Hyewon Seo2, Jihoon Lee2
1KNU G-LAMP Project Group, KNU Institute of Basic Sciences, Department of Chemistry, Kyungpook National University, Daegu 41566, Republic of Korea.
Abstract:
The β-amino ketones produced through the Mannich reaction hold significant potential as candidates for various drugs. In this study, we optimized on-DNA Mannich reaction conditions and applied them to investigate the reactions of DNA-conjugated aldehydes with various amine and ketone building blocks. The developed on-DNA Mannich reaction preserved the DNA integrity and established viable routes for library production. These results underscore the potential of the Mannich reaction in DNA-encoded library (DEL) synthesis.
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