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Updated: May 12, 2026

A Whole Mount In Situ Hybridization Method for the Gastropod Mollusc Lymnaea stagnalis
Published on: March 15, 2016
Lutz's spontaneous sedimentation technique and the paleoparasitological analysis of sambaqui (shell mound) sediments
Morgana Camacho1, Thaíla Pessanha, Daniela Leles
1Laboratório de Paleoparasitologia, Departamento de Endemias Samuel Pessoa, Escola Nacional de Saúde Pública Sergio Arouca, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, RJ, Brasil.
Abstract:
Parasite findings in sambaquis (shell mounds) are scarce. Although the 121 shell mound samples were previously analysed in our laboratory, we only recently obtained the first positive results. In the sambaqui of Guapi, Rio de Janeiro, Brazil, paleoparasitological analysis was performed on sediment samples collected from various archaeological layers, including the superficial layer as a control. Eggs of Acanthocephala, Ascaridoidea and Heterakoidea were found in the archaeological layers. We applied various techniques and concluded that Lutz's spontaneous sedimentation technique is effective for concentrating parasite eggs in sambaqui soil for microscopic analysis.

