Replacement of harmful animal use in life science education: the approach and activities of InterNICHE

Nick Jukes1

  • 1InterNICHE (International Network for Humane Education), 19 Brookhouse Avenue, Leicester LE2 0JE, UK. coordinator@interniche.org

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Integrins01:10

Integrins

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Activation of Integrins01:15

Activation of Integrins

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In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

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iChip01:24

iChip

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