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Evaluation of cytotoxic cell-target cell conjugates. Comparison between flow cytometric analysis and 51Cr binding
S Heltai1, A Poggi, M G Sabbadini
1Istituto Scientifico San Raffaele, Milano, Italy.
Haematologica
|March 1, 1990
Abstract:
Two methods for evaluating the conjugate formation between either bulk cultured or cloned NK cells and fresh tumor target cells or tumor cell lines were compared. Flow cytometric analysis and 51Cr binding assay yielded comparable results using NK clones as effector cells; however, flow cytometry provides less accuracy using large granular lymphocytes or unfractionated lymphocytes.