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Related Concept Videos

Mechanism of Angiogenesis01:10

Mechanism of Angiogenesis

Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
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Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...
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Modified-Release Drug Delivery Systems: Site-Targeted

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Site-Targeted Drug Delivery Systems: Polymeric Carriers01:24

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Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...
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The selection of a drug's delivery route depends upon its physicochemical properties, including lipid or water solubility and ionization, as well as the therapeutic requirement, such as immediate or sustained effect. These routes can be divided into three primary categories: enteral, parenteral, and topical.
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Programming Stem Cells for Therapeutic Angiogenesis Using Biodegradable Polymeric Nanoparticles
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Published on: September 27, 2013

Dual growth factor delivery using biocompatible core-shell microcapsules for angiogenesis.

Dong Hoon Choi1, Ramesh Subbiah, Ik Hwan Kim

  • 1Center for Biomaterials, Korea Institute of Science and Technology (KIST), Seoul 136-791, Republic of Korea; Department of Biological Science, Korea University, Seoul 136-701, Republic of Korea.

Small (Weinheim an Der Bergstrasse, Germany)
|April 16, 2013
PubMed
Summary

This study optimized core-shell microcapsules (C-S MCs) using PLGA and alginate for controlled release of proteins and growth factors. The system demonstrated enhanced angiogenic potential in endothelial cells, highlighting adjustable biomolecule delivery.

Keywords:
angiogenesiscore/shell materialselectrodroppinggrowth factorsmicrocapsules

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Area of Science:

  • Biomaterials Science
  • Drug Delivery Systems
  • Tissue Engineering

Background:

  • Controlled release of therapeutic biomolecules is crucial for effective treatments.
  • Microcapsule systems offer potential for encapsulating and delivering sensitive molecules.
  • Tailoring microcapsule properties can modulate release kinetics and biological activity.

Purpose of the Study:

  • To develop and optimize a core-shell microcapsule (C-S MC) system for controlled release of proteins and angiogenic growth factors.
  • To investigate the impact of polymer properties (PLGA molecular weight) and surface modifications (layer-by-layer assembly) on release profiles.
  • To evaluate the angiogenic potential of growth factors delivered via C-S MCs in vitro.

Main Methods:

  • Fabrication of homogeneous C-S MCs using an electrodropping system with poly(L-lactic-co-glycolic acid) (PLGA) and alginate.
  • Characterization of C-S MCs using fluorescence imaging.
  • Assessment of protein and growth factor release profiles under varying PLGA molecular weights and layer-by-layer assembly conditions.
  • In vitro evaluation of angiogenic activity using human umbilical vein endothelial cells (HUVECs) and CD31+ staining.

Main Results:

  • Optimized electrodropping produced homogeneous C-S MCs with distinct core-shell domains.
  • High-molecular-weight PLGA and 7-layer LBL assembly significantly reduced initial burst release of proteins compared to low-MW PLGA and no LBL.
  • Differential release rates were observed for co-encapsulated growth factors (VEGF vs. PDGF), influenced by core PLGA type.
  • MC-mediated growth factor delivery significantly enhanced angiogenic sprouting in HUVECs compared to conventional delivery.

Conclusions:

  • Biocompatible C-S MCs can be fabricated with tunable release characteristics for biomolecules.
  • PLGA molecular weight and LBL assembly provide effective control over release kinetics, minimizing initial burst release.
  • MC-encapsulated growth factors retain activity and promote significant angiogenic responses in endothelial cells.