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Related Experiment Video

Updated: Apr 10, 2026

Author Spotlight: Optimizing Bovine Lung Decellularization for Organotypic Hydrogels
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Thermoreversible cell-derived extracellular matrix only hydrogel (CEOgel): Development, characterization, and

Byoungha An1,2, Jae Won Kwon1, Heejeong Yoon3

  • 1Center for Biomaterials, Korea Institute of Science and Technology (KIST), Seoul, 02792, Republic of Korea.

Materials Today. Bio
|April 9, 2026
PubMed
Summary

Researchers developed CEOgel, a novel thermoreversible hydrogel made entirely from cell-derived extracellular matrix (cECM). This biomaterial supports cell growth and tissue regeneration without animal products or external cross-linkers.

Keywords:
Cell-derived extracellular matrixDecellularized extracellular matrixECM hydrogelThermoreversibleThermosensitive

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Decellularized extracellular matrix (dECM) is crucial for 3D cell culture and tissue regeneration.
  • Cell-derived ECM (cECM) offers an alternative to tissue-derived dECM, but thermosensitive hydrogels are underexplored.
  • Existing cECM formulations lack thermoreversible properties.

Purpose of the Study:

  • To develop a novel thermoreversible hydrogel exclusively from cell-derived extracellular matrix (cECM).
  • To characterize the properties and potential applications of this new biomaterial, termed CEOgel.
  • To explore tunable properties and expand the design diversity of ECM hydrogels.

Main Methods:

  • Decellularization of in vitro-cultured umbilical cord mesenchymal stem cells to enhance cECM solubility.
  • Concentration of cECM nanofibers to create CEOgel without additional factors.
  • Characterization of CEOgel's mechanical stability, thermosensitivity, biocompatibility, and protein composition via proteomics.

Main Results:

  • CEOgel is a thermoreversible hydrogel formed solely from cECM, applicable across multiple cell types with minimal variability.
  • The hydrogel demonstrates mechanical stability for in vitro use and in vivo gelation, confirming thermosensitivity and biocompatibility.
  • CEOgel supports endothelial vascularization and cancer organoid growth, containing a broad spectrum of human tissue proteins.

Conclusions:

  • CEOgel represents a new class of thermoreversible cECM hydrogels, eliminating the need for animal tissues or external cross-linkers.
  • This biomaterial expands the utility of cECM and advances ECM hydrogel design for regenerative medicine.
  • CEOgel shows significant potential as a regenerative biomaterial for tissue engineering and various medical applications.