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Capillary Electrophoresis: Applications

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

Capillary Electrophoresis: Instrumentation

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...

Electrophoresis: Overview

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...

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Related Experiment Video

Updated: May 12, 2026

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Published on: November 21, 2023

Microfab-less Microfluidic Capillary Electrophoresis Devices.

Thiago P Segato¹, Samir A Bhakta, Matthew Gordon

¹Instituto de Quimica de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil.

Analytical Methods : Advancing Methods and Applications

|April 16, 2013

Summary

A novel hybrid microfluidic platform offers versatile, rapid, and inexpensive analysis. This new 5^2-platform enables quick assembly for capillary electrophoresis, ideal for field applications like analyzing Atacama Desert soil samples.

Keywords:

PMMA conductivity detection fabrication inorganic ions microchip

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Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets

Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets

Published on: February 22, 2016

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Last Updated: May 12, 2026

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Published on: November 21, 2023

Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets

Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets

Published on: February 22, 2016

Area of Science:

Analytical Chemistry
Microfluidics

Background:

Microfluidic devices offer portability and speed but face fabrication challenges.
Traditional methods often use materials with poor surface properties and complex procedures.

Purpose of the Study:

To introduce a versatile hybrid microfluidic platform (5^2-platform) overcoming fabrication limitations.
To demonstrate the platform's utility for rapid analysis of complex samples.

Main Methods:

A novel platform using 5 interconnecting plastic microfluidic components and standard capillary tubing.
Fabrication via inexpensive engraving tools and Plexiglas.
Capacitively-coupled contactless conductivity detection (C4D) for in-channel detection.

Main Results:

The 5^2-platform allows assembly in minutes with versatile capillary tubing options.
Minimum impact on separation efficiency was observed.
Successful analysis of inorganic cations in Atacama Desert soil samples using capillary electrophoresis.

Conclusions:

The 5^2-platform provides a versatile, cost-effective, and rapidly assembled solution for microfluidic analysis.
This approach simplifies microfluidic device fabrication and enhances portability.
Demonstrates potential for field analysis of environmental samples.